Expanding the scope of quantitative FRAP analysis.

Published

Journal Article

In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in conical geometry, a fast computational method for calculation of fluorescence recovery is presented. Two approximations are presented to aid in FRAP analysis when binding varies spatially, one applying to cases of relatively fast diffusion and slow binding and the other to binding of molecules to small cellular structures. Numerical results show that using a model that represents the influential physical processes and that is formulated in the appropriate geometry can substantially improve the accuracy of FRAP calculations.

Full Text

Duke Authors

Cited Authors

  • Hallen, MA; Layton, AT

Published Date

  • January 2010

Published In

Volume / Issue

  • 262 / 2

Start / End Page

  • 295 - 305

PubMed ID

  • 19836405

Pubmed Central ID

  • 19836405

Electronic International Standard Serial Number (EISSN)

  • 1095-8541

International Standard Serial Number (ISSN)

  • 0022-5193

Digital Object Identifier (DOI)

  • 10.1016/j.jtbi.2009.10.020

Language

  • eng