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Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk.

Publication ,  Journal Article
Ramos, IB; Miranda, K; Ulrich, P; Ingram, P; LeFurgey, A; Machado, EA; de Souza, W; Docampo, R
Published in: Biol Cell
April 9, 2010

BACKGROUND INFORMATION: Poly P (inorganic polyphosphate) is a polymer formed by P(i) residues linked by high-energy phosphoanhydride bonds. The presence of poly P in bacteria, fungi, algae and protists has been widely recognized, but the distribution of poly P in more complex eukaryotes has been poorly studied. Poly P accumulates, together with calcium, in acidic vesicles or acidocalcisomes in a number of organisms and possesses a diverse array of functions, including roles in stress response, blood clotting, inflammation, calcification, cell proliferation and apoptosis. RESULTS: We report here that a considerable amount of phosphorus in the yolk of chicken eggs is in the form of poly P. DAPI (4',6-diamidino-2-phenylindole) staining showed that poly P is localized mainly in electron-dense vesicles located inside larger vacuoles (compound organelles) that are randomly distributed in the yolk. These internal vesicles were shown to contain calcium, potassium, sodium, magnesium, phosphorus, chlorine, iron and zinc, as detected by X-ray microanalysis and elemental mapping. These vesicles stain with the acidophilic dye Acridine Orange. The presence of poly P in organellar fractions of the egg yolk was evident in agarose gels stained with Toluidine Blue and DAPI. Of the total phosphate (Pi) of yolk organelles, 16% is present in the form of poly P. Total poly P content was not altered during the first 4 days of embryogenesis, but poly P chain length decreased after 1 day of development. CONCLUSIONS: The results of the present study identify a novel organelle in chicken egg yolk comprising acidic vesicles with a morphology, physiology and composition similar to those of acidocalcisomes, within larger acidic vacuoles. The elemental composition of these acidocalcisomes is proportionally similar to the elemental composition of the yolk, suggesting that most of these elements are located in these organelles, which might be an important storage compartment in eggs.

Duke Scholars

Published In

Biol Cell

DOI

EISSN

1768-322X

Publication Date

April 9, 2010

Volume

102

Issue

7

Start / End Page

421 / 434

Location

England

Related Subject Headings

  • Vacuolar Proton-Translocating ATPases
  • Polyphosphates
  • Macrolides
  • Intracellular Membranes
  • Embryonic Development
  • Electron Probe Microanalysis
  • Egg Yolk
  • Developmental Biology
  • Cytoplasmic Vesicles
  • Chickens
 

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Ramos, I. B., Miranda, K., Ulrich, P., Ingram, P., LeFurgey, A., Machado, E. A., … Docampo, R. (2010). Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk. Biol Cell, 102(7), 421–434. https://doi.org/10.1042/BC20100011
Ramos, Isabela B., Kildare Miranda, Paul Ulrich, Peter Ingram, Ann LeFurgey, Ednildo A. Machado, Wanderley de Souza, and Roberto Docampo. “Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk.Biol Cell 102, no. 7 (April 9, 2010): 421–34. https://doi.org/10.1042/BC20100011.
Ramos IB, Miranda K, Ulrich P, Ingram P, LeFurgey A, Machado EA, et al. Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk. Biol Cell. 2010 Apr 9;102(7):421–34.
Ramos, Isabela B., et al. “Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk.Biol Cell, vol. 102, no. 7, Apr. 2010, pp. 421–34. Pubmed, doi:10.1042/BC20100011.
Ramos IB, Miranda K, Ulrich P, Ingram P, LeFurgey A, Machado EA, de Souza W, Docampo R. Calcium- and polyphosphate-containing acidocalcisomes in chicken egg yolk. Biol Cell. 2010 Apr 9;102(7):421–434.
Journal cover image

Published In

Biol Cell

DOI

EISSN

1768-322X

Publication Date

April 9, 2010

Volume

102

Issue

7

Start / End Page

421 / 434

Location

England

Related Subject Headings

  • Vacuolar Proton-Translocating ATPases
  • Polyphosphates
  • Macrolides
  • Intracellular Membranes
  • Embryonic Development
  • Electron Probe Microanalysis
  • Egg Yolk
  • Developmental Biology
  • Cytoplasmic Vesicles
  • Chickens