Time course of calcium deposition in shells of the barnacle (Balanus amphitrite amphitrite) during cyprid-juvenile metamorphosis
Light microscopy and scanning electron microscopy were used to study the morphology of balanomorph barnacles during metamorphosis from the cyprid larval stage to the juvenile. First, laboratory-raised, free-swimming cyprid larvae were allowed to settle on plastic coverslips in culture dishes of seawater. Then, the cyprids were cryopreserved, freeze dried, rotary carbon-coated and examined. Finally, electron probe X-ray microanalysis dot maps were obtained.
Wallace, NR; Freudenrich, CC; Wilbur, K; Ingram, P; LeFurgey, A
Proceedings Annual Meeting, Microscopy Society of America
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