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Suspension of medullary thick ascending limb tubules from the rabbit kidney

Publication ,  Journal Article
Chamberlin, ME; LeFurgey, A; Mandel, LJ
Published in: American Journal of Physiology - Renal Fluid and Electrolyte Physiology
December 1, 1984

A procedure for isolating a suspension of tubules derived from rabbit medullary thick ascending limb is described. The purity of the preparation was assessed by microscopy and enzyme assays and the viability of the preparation was assessed by measuring oxygen consumption. Microscopy revealed that the suspension contains 95% thick ascending limbs and that the isolation procedure preserves the structure of the epithelium except for the loss of the basement membrane. The preparation had a high activity of calcitonin-sensitive adenylate cyclase, a marker enzyme for the medullary thick ascending limb. Control oxygen consumption was considerably higher than that reported for proximal tubules in the literature, and nystatin or carbonyl cyanide p-trifluoromethoxyphenylhydrazone addition produced a more than 100% increase in oxygen consumption. Furosemide inhibited the oxygen consumption by 43% and ouabain inhibited it by 42%. Furosemide inhibited sodium chloride entry without directly affecting the Na-K-ATPase or cellular metabolism. Chloride removal depressed oxygen consumption to the same extent as furosemide, but some of this action was through direct inhibition of cellular metabolism.

Duke Scholars

Published In

American Journal of Physiology - Renal Fluid and Electrolyte Physiology

Publication Date

December 1, 1984

Volume

16

Issue

6

Related Subject Headings

  • Urology & Nephrology
  • 1116 Medical Physiology
  • 1103 Clinical Sciences
  • 0606 Physiology
 

Citation

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MLA
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Chamberlin, M. E., LeFurgey, A., & Mandel, L. J. (1984). Suspension of medullary thick ascending limb tubules from the rabbit kidney. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 16(6).
Chamberlin, M. E., A. LeFurgey, and L. J. Mandel. “Suspension of medullary thick ascending limb tubules from the rabbit kidney.” American Journal of Physiology - Renal Fluid and Electrolyte Physiology 16, no. 6 (December 1, 1984).
Chamberlin ME, LeFurgey A, Mandel LJ. Suspension of medullary thick ascending limb tubules from the rabbit kidney. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1984 Dec 1;16(6).
Chamberlin, M. E., et al. “Suspension of medullary thick ascending limb tubules from the rabbit kidney.” American Journal of Physiology - Renal Fluid and Electrolyte Physiology, vol. 16, no. 6, Dec. 1984.
Chamberlin ME, LeFurgey A, Mandel LJ. Suspension of medullary thick ascending limb tubules from the rabbit kidney. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1984 Dec 1;16(6).

Published In

American Journal of Physiology - Renal Fluid and Electrolyte Physiology

Publication Date

December 1, 1984

Volume

16

Issue

6

Related Subject Headings

  • Urology & Nephrology
  • 1116 Medical Physiology
  • 1103 Clinical Sciences
  • 0606 Physiology