Robust control of mitotic spindle orientation in the developing epidermis.

Journal Article (Journal Article)

Progenitor cells must balance self-amplification and production of differentiated progeny during development and homeostasis. In the epidermis, progenitors divide symmetrically to increase surface area and asymmetrically to promote stratification. In this study, we show that individual epidermal cells can undergo both types of division, and therefore, the balance is provided by the sum of individual cells' choices. In addition, we define two control points for determining a cell's mode of division. First is the expression of the mouse Inscuteable gene, which is sufficient to drive asymmetric cell division (ACD). However, there is robust control of division orientation as excessive ACDs are prevented by a change in the localization of NuMA, an effector of spindle orientation. Finally, we show that p63, a transcriptional regulator of stratification, does not control either of these processes. These data have uncovered two important regulatory points controlling ACD in the epidermis and allow a framework for analysis of how external cues control this important choice.

Full Text

Duke Authors

Cited Authors

  • Poulson, ND; Lechler, T

Published Date

  • November 29, 2010

Published In

Volume / Issue

  • 191 / 5

Start / End Page

  • 915 - 922

PubMed ID

  • 21098114

Pubmed Central ID

  • PMC2995176

Electronic International Standard Serial Number (EISSN)

  • 1540-8140

Digital Object Identifier (DOI)

  • 10.1083/jcb.201008001


  • eng

Conference Location

  • United States