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Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators.

Publication ,  Journal Article
Yan, D; Cho, HS; Hastings, CA; Igo, MM; Lee, SY; Pelton, JG; Stewart, V; Wemmer, DE; Kustu, S
Published in: Proc Natl Acad Sci U S A
December 21, 1999

Two-component systems, sensor kinase-response regulator pairs, dominate bacterial signal transduction. Regulation is exerted by phosphorylation of an Asp in receiver domains of response regulators. Lability of the acyl phosphate linkage has limited structure determination for the active, phosphorylated forms of receiver domains. As assessed by both functional and structural criteria, beryllofluoride yields an excellent analogue of aspartyl phosphate in response regulator NtrC, a bacterial enhancer-binding protein. Beryllofluoride also appears to activate the chemotaxis, sporulation, osmosensing, and nitrate/nitrite response regulators CheY, Spo0F, OmpR, and NarL, respectively. NMR spectroscopic studies indicate that beryllofluoride will facilitate both biochemical and structural characterization of the active forms of receiver domains.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 21, 1999

Volume

96

Issue

26

Start / End Page

14789 / 14794

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Protein Binding
  • Phosphorylation
  • Phosphoproteins
  • PII Nitrogen Regulatory Proteins
  • Nuclear Magnetic Resonance, Biomolecular
  • Methyl-Accepting Chemotaxis Proteins
  • Membrane Proteins
 

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Yan, D., Cho, H. S., Hastings, C. A., Igo, M. M., Lee, S. Y., Pelton, J. G., … Kustu, S. (1999). Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci U S A, 96(26), 14789–14794. https://doi.org/10.1073/pnas.96.26.14789
Yan, D., H. S. Cho, C. A. Hastings, M. M. Igo, S. Y. Lee, J. G. Pelton, V. Stewart, D. E. Wemmer, and S. Kustu. “Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators.Proc Natl Acad Sci U S A 96, no. 26 (December 21, 1999): 14789–94. https://doi.org/10.1073/pnas.96.26.14789.
Yan D, Cho HS, Hastings CA, Igo MM, Lee SY, Pelton JG, et al. Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14789–94.
Yan, D., et al. “Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators.Proc Natl Acad Sci U S A, vol. 96, no. 26, Dec. 1999, pp. 14789–94. Pubmed, doi:10.1073/pnas.96.26.14789.
Yan D, Cho HS, Hastings CA, Igo MM, Lee SY, Pelton JG, Stewart V, Wemmer DE, Kustu S. Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14789–14794.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 21, 1999

Volume

96

Issue

26

Start / End Page

14789 / 14794

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Protein Binding
  • Phosphorylation
  • Phosphoproteins
  • PII Nitrogen Regulatory Proteins
  • Nuclear Magnetic Resonance, Biomolecular
  • Methyl-Accepting Chemotaxis Proteins
  • Membrane Proteins