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G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition.

Publication ,  Journal Article
Diverse-Pierluissi, M; Inglese, J; Stoffel, RH; Lefkowitz, RJ; Dunlap, K
Published in: Neuron
March 1996

G protein-coupled receptors are essential signaling molecules at sites of synaptic transmission. Here, we explore the mechanisms responsible for the use-dependent termination of metabotropic receptor signaling in embryonic sensory neurons. We report that the inhibition of voltage-dependent Ca2+ channels mediated by alpha2-adrenergic receptors desensitizes slowly with prolonged exposure to the transmitter and that the desensitization is mediated by a G protein-coupled receptor kinase (GRK). Intracellular introduction of recombinant, purified kinases or synthetic blocking peptides into individual neurons demonstrates the specific involvement of a GRK3-like protein. These results suggest that GRK-mediated termination of receptor-G protein coupling is likely to regulate synaptic strength and, as such, may provide one effective mechanism for depression of synaptic transmission.

Duke Scholars

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

March 1996

Volume

16

Issue

3

Start / End Page

579 / 585

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Protein Kinases
  • Phosphorylation
  • Patch-Clamp Techniques
  • Norepinephrine
  • Neurology & Neurosurgery
  • GTP-Binding Proteins
  • Dose-Response Relationship, Drug
  • Chick Embryo
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Diverse-Pierluissi, M., Inglese, J., Stoffel, R. H., Lefkowitz, R. J., & Dunlap, K. (1996). G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition. Neuron, 16(3), 579–585. https://doi.org/10.1016/s0896-6273(00)80077-x
Diverse-Pierluissi, M., J. Inglese, R. H. Stoffel, R. J. Lefkowitz, and K. Dunlap. “G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition.Neuron 16, no. 3 (March 1996): 579–85. https://doi.org/10.1016/s0896-6273(00)80077-x.
Diverse-Pierluissi M, Inglese J, Stoffel RH, Lefkowitz RJ, Dunlap K. G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition. Neuron. 1996 Mar;16(3):579–85.
Diverse-Pierluissi, M., et al. “G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition.Neuron, vol. 16, no. 3, Mar. 1996, pp. 579–85. Pubmed, doi:10.1016/s0896-6273(00)80077-x.
Diverse-Pierluissi M, Inglese J, Stoffel RH, Lefkowitz RJ, Dunlap K. G protein-coupled receptor kinase mediates desensitization of norepinephrine-induced Ca2+ channel inhibition. Neuron. 1996 Mar;16(3):579–585.
Journal cover image

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

March 1996

Volume

16

Issue

3

Start / End Page

579 / 585

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Protein Kinases
  • Phosphorylation
  • Patch-Clamp Techniques
  • Norepinephrine
  • Neurology & Neurosurgery
  • GTP-Binding Proteins
  • Dose-Response Relationship, Drug
  • Chick Embryo
  • Cells, Cultured