A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits.

Published

Journal Article

Receptor-mediated activation of heterotrimeric guanine nucleotide-binding proteins (G proteins) results in the dissociation of alpha from beta gamma subunits, thereby allowing both to regulate effectors. Little is known about the regions of effectors required for recognition of G beta gamma. A peptide encoding residues 956 to 982 of adenylyl cyclase 2 specifically blocked G beta gamma stimulation of adenylyl cyclase 2, phospholipase C-beta 3, potassium channels, and beta-adrenergic receptor kinase as well as inhibition of calmodulin-stimulated adenylyl cyclases, but had no effect on interactions between G beta gamma and G alpha o. Substitutions in this peptide identified a functionally important motif, Gln-X-X-Glu-Arg, that is also conserved in regions of potassium channels and beta-adrenergic receptor kinases that participate in G beta gamma interactions. Thus, the region defined by residues 956 to 982 of adenylyl cyclase 2 may contain determinants important for receiving signals from G beta gamma.

Full Text

Duke Authors

Cited Authors

  • Chen, J; DeVivo, M; Dingus, J; Harry, A; Li, J; Sui, J; Carty, DJ; Blank, JL; Exton, JH; Stoffel, RH

Published Date

  • May 1995

Published In

Volume / Issue

  • 268 / 5214

Start / End Page

  • 1166 - 1169

PubMed ID

  • 7761832

Pubmed Central ID

  • 7761832

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.7761832

Language

  • eng