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Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229.

Publication ,  Journal Article
Regan, JW; Raymond, JR; Lefkowitz, RJ; DeMarinis, RM
Published in: Biochem Biophys Res Commun
June 13, 1986

A newly developed alpha 2-adrenergic photoaffinity ligand, 3-methyl-6-chloro-9-azido-1H-2,3,4,5-tetrahydro-3-benzazepine (SKF 102229), has been radiolabeled with tritium to a specific activity of approximately 80 Ci/mmol. Using membranes prepared from human platelets and from rabbit kidney, alpha 2-adrenoceptors have been covalently labeled following photolysis in the presence of [3H]SKF 102229. As determined by SDS-PAGE, the apparent molecular weight of alpha 2-adrenoceptors from both of these tissues was 64,000. The yield of covalent insertion of [3H]SKF 102229 into the alpha 2-adrenoceptor was very good. Thus, following photolysis up to 90% of the alpha 2-adrenoceptors could be irreversibly labeled with [3H]SKF 102229.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 13, 1986

Volume

137

Issue

2

Start / End Page

606 / 613

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Receptors, Adrenergic, alpha
  • Rabbits
  • Photochemistry
  • Kidney
  • Humans
  • Electrophoresis, Polyacrylamide Gel
  • Blood Platelets
  • Biochemistry & Molecular Biology
  • Benzazepines
 

Citation

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Regan, J. W., Raymond, J. R., Lefkowitz, R. J., & DeMarinis, R. M. (1986). Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229. Biochem Biophys Res Commun, 137(2), 606–613. https://doi.org/10.1016/0006-291x(86)91121-6
Regan, J. W., J. R. Raymond, R. J. Lefkowitz, and R. M. DeMarinis. “Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229.Biochem Biophys Res Commun 137, no. 2 (June 13, 1986): 606–13. https://doi.org/10.1016/0006-291x(86)91121-6.
Regan JW, Raymond JR, Lefkowitz RJ, DeMarinis RM. Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229. Biochem Biophys Res Commun. 1986 Jun 13;137(2):606–13.
Regan, J. W., et al. “Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229.Biochem Biophys Res Commun, vol. 137, no. 2, June 1986, pp. 606–13. Pubmed, doi:10.1016/0006-291x(86)91121-6.
Regan JW, Raymond JR, Lefkowitz RJ, DeMarinis RM. Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229. Biochem Biophys Res Commun. 1986 Jun 13;137(2):606–613.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 13, 1986

Volume

137

Issue

2

Start / End Page

606 / 613

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Receptors, Adrenergic, alpha
  • Rabbits
  • Photochemistry
  • Kidney
  • Humans
  • Electrophoresis, Polyacrylamide Gel
  • Blood Platelets
  • Biochemistry & Molecular Biology
  • Benzazepines