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Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes.

Publication ,  Journal Article
Sibley, DR; Nambi, P; Peters, JR; Lefkowitz, RJ
Published in: Biochem Biophys Res Commun
June 29, 1984

Preincubation of duck erythrocytes with tumor promoting phorbol diesters or catecholamines leads to attenuation of adenylate cyclase activity. 12-0-Tetradecanoyl phorbol-13-acetate (TPA) and phorbol 12,13-dibutyrate treatment induced a 38% and 30% desensitization of isoproterenol-stimulated adenylate cyclase activity, respectively. In contrast, the inactive phorbol diester, 4 alpha-phorbol 12,13-didecanoate, was without effect in promoting adenylate cyclase desensitization. The catecholamine isoproterenol induced a 51% desensitization. Incubation of 32Pi labeled erythrocytes with TPA promoted a 3- to 4-fold increase in phosphorylation of the beta-adrenergic receptor as did incubation with isoproterenol. Treatment of the cells with both TPA and isoproterenol together resulted in desensitization and receptor phosphorylation which were no greater than those observed with either agent alone. These data suggest a potential role for protein kinase C in regulating beta-adrenergic receptor function.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 29, 1984

Volume

121

Issue

3

Start / End Page

973 / 979

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Photochemistry
  • Phosphorylation
  • Phorbols
  • Phorbol Esters
  • Isoproterenol
  • Erythrocytes
  • Electrophoresis, Polyacrylamide Gel
  • Ducks
  • Biochemistry & Molecular Biology
 

Citation

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Sibley, D. R., Nambi, P., Peters, J. R., & Lefkowitz, R. J. (1984). Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes. Biochem Biophys Res Commun, 121(3), 973–979. https://doi.org/10.1016/0006-291x(84)90772-1
Sibley, D. R., P. Nambi, J. R. Peters, and R. J. Lefkowitz. “Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes.Biochem Biophys Res Commun 121, no. 3 (June 29, 1984): 973–79. https://doi.org/10.1016/0006-291x(84)90772-1.
Sibley DR, Nambi P, Peters JR, Lefkowitz RJ. Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes. Biochem Biophys Res Commun. 1984 Jun 29;121(3):973–9.
Sibley, D. R., et al. “Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes.Biochem Biophys Res Commun, vol. 121, no. 3, June 1984, pp. 973–79. Pubmed, doi:10.1016/0006-291x(84)90772-1.
Sibley DR, Nambi P, Peters JR, Lefkowitz RJ. Phorbol diesters promote beta-adrenergic receptor phosphorylation and adenylate cyclase desensitization in duck erythrocytes. Biochem Biophys Res Commun. 1984 Jun 29;121(3):973–979.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 29, 1984

Volume

121

Issue

3

Start / End Page

973 / 979

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Photochemistry
  • Phosphorylation
  • Phorbols
  • Phorbol Esters
  • Isoproterenol
  • Erythrocytes
  • Electrophoresis, Polyacrylamide Gel
  • Ducks
  • Biochemistry & Molecular Biology