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Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes.

Publication ,  Journal Article
Stiles, GL; Stadel, JM; De Lean, A; Lefkowitz, RJ
Published in: J Clin Invest
December 1981

We have investigated alterations in beta adrenergic receptor binding sites of rat reticulocytes occurring in animals rendered hypothyroid by thyroidectomy. Beta adrenergic receptor interactions were assessed by measuring the number of (-)[3H]-dihydroalprenolol binding sites and the ability of an agonist to compete for occupancy of the receptors. The number of receptors was significantly reduced in cells from the hypothyroid animals. In addition, there were significant agonist-specific alterations in binding. Using computer assisted curve fitting techniques, it was found that the ability of (-)isoproterenol to stabilize a high affinity guanine nucleotide sensitive "coupled" form of the receptor was impaired. Reticulocytes from hypothyroid animals have, in addition, a reduction in the concentration of the nucleotide regulatory protein as assessed by the number of 42,000 Mr substrates for cholera toxin catalyzed ADP ribosylation. These alterations are associated with reductions in catecholamine and NaF stimulated adenylate cyclase activity. Diminished coupling of beta adrenergic receptors with other regulatory components of the adenylate cyclase system represents a mechanism by which altered thyroid states modulate beta adrenergic receptor function and beta adrenergic responsiveness of tissues.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

December 1981

Volume

68

Issue

6

Start / End Page

1450 / 1455

Location

United States

Related Subject Headings

  • Thyroidectomy
  • Reticulocytes
  • Receptors, Cell Surface
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats, Inbred Strains
  • Rats
  • Protein Binding
  • Models, Biological
  • Isoproterenol
 

Citation

APA
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ICMJE
MLA
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Stiles, G. L., Stadel, J. M., De Lean, A., & Lefkowitz, R. J. (1981). Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes. J Clin Invest, 68(6), 1450–1455. https://doi.org/10.1172/jci110397
Stiles, G. L., J. M. Stadel, A. De Lean, and R. J. Lefkowitz. “Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes.J Clin Invest 68, no. 6 (December 1981): 1450–55. https://doi.org/10.1172/jci110397.
Stiles GL, Stadel JM, De Lean A, Lefkowitz RJ. Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes. J Clin Invest. 1981 Dec;68(6):1450–5.
Stiles, G. L., et al. “Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes.J Clin Invest, vol. 68, no. 6, Dec. 1981, pp. 1450–55. Pubmed, doi:10.1172/jci110397.
Stiles GL, Stadel JM, De Lean A, Lefkowitz RJ. Hypothyroidism modulates beta adrenergic receptor adenylate cyclase interactions in rat reticulocytes. J Clin Invest. 1981 Dec;68(6):1450–1455.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

December 1981

Volume

68

Issue

6

Start / End Page

1450 / 1455

Location

United States

Related Subject Headings

  • Thyroidectomy
  • Reticulocytes
  • Receptors, Cell Surface
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats, Inbred Strains
  • Rats
  • Protein Binding
  • Models, Biological
  • Isoproterenol