Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes.

Published

Journal Article

Desensitization of catecholamine stimulated adenylate cyclase (AC) activity is demonstrated in membranes derived from turkey erythrocytes pre-treated with isoproterenol. Membranes from desensitized cells had a loss in maximal catecholamine stimulated adenylate cyclase activity of 104 +/- 13 (pmols/mg protein/10', p less than .001) compared with controls. When adenylate cyclase was maximally stimulated with NaF or Gpp(NH)p, the decrements were 84 +/- 19 (p less than .005) and 92 +/- 32 (p less than .05) pmol/mg protein/10' respectively. There was no change in beta-adrenergic receptor number in membranes derived from treated cells. While the molecular mechanism accounting for the desensitization is uncertain, the data is consistent with the hypothesis that there is a lesion distal to the beta-adrenergic receptor, possibly involving the nucleotide site or the catalytic subunit of adenylate cyclase, causing the desensitization in the isoproterenol treated cells.

Full Text

Duke Authors

Cited Authors

  • Hoffman, BB; Mullikin-Kilpatrick, D; Lefkowitz, RJ

Published Date

  • October 1, 1979

Published In

Volume / Issue

  • 5 / 5

Start / End Page

  • 355 - 366

PubMed ID

  • 230209

Pubmed Central ID

  • 230209

International Standard Serial Number (ISSN)

  • 0095-1544

Language

  • eng