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Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes.

Publication ,  Journal Article
Hoffman, BB; Mullikin-Kilpatrick, D; Lefkowitz, RJ
Published in: J Cyclic Nucleotide Res
October 1979

Desensitization of catecholamine stimulated adenylate cyclase (AC) activity is demonstrated in membranes derived from turkey erythrocytes pre-treated with isoproterenol. Membranes from desensitized cells had a loss in maximal catecholamine stimulated adenylate cyclase activity of 104 +/- 13 (pmols/mg protein/10', p less than .001) compared with controls. When adenylate cyclase was maximally stimulated with NaF or Gpp(NH)p, the decrements were 84 +/- 19 (p less than .005) and 92 +/- 32 (p less than .05) pmol/mg protein/10' respectively. There was no change in beta-adrenergic receptor number in membranes derived from treated cells. While the molecular mechanism accounting for the desensitization is uncertain, the data is consistent with the hypothesis that there is a lesion distal to the beta-adrenergic receptor, possibly involving the nucleotide site or the catalytic subunit of adenylate cyclase, causing the desensitization in the isoproterenol treated cells.

Duke Scholars

Published In

J Cyclic Nucleotide Res

ISSN

0095-1544

Publication Date

October 1979

Volume

5

Issue

5

Start / End Page

355 / 366

Location

United States

Related Subject Headings

  • Turkeys
  • Stimulation, Chemical
  • Receptors, Adrenergic, beta
  • Propranolol
  • Isoproterenol
  • Erythrocytes
  • Erythrocyte Membrane
  • Enzyme Activation
  • Animals
  • Adenylyl Cyclases
 

Citation

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Hoffman, B. B., Mullikin-Kilpatrick, D., & Lefkowitz, R. J. (1979). Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes. J Cyclic Nucleotide Res, 5(5), 355–366.
Hoffman, B. B., D. Mullikin-Kilpatrick, and R. J. Lefkowitz. “Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes.J Cyclic Nucleotide Res 5, no. 5 (October 1979): 355–66.
Hoffman BB, Mullikin-Kilpatrick D, Lefkowitz RJ. Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes. J Cyclic Nucleotide Res. 1979 Oct;5(5):355–66.
Hoffman, B. B., et al. “Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes.J Cyclic Nucleotide Res, vol. 5, no. 5, Oct. 1979, pp. 355–66.
Hoffman BB, Mullikin-Kilpatrick D, Lefkowitz RJ. Desensitization of beta-adrenergic stimulated adenylate cyclase in turkey erythrocytes. J Cyclic Nucleotide Res. 1979 Oct;5(5):355–366.

Published In

J Cyclic Nucleotide Res

ISSN

0095-1544

Publication Date

October 1979

Volume

5

Issue

5

Start / End Page

355 / 366

Location

United States

Related Subject Headings

  • Turkeys
  • Stimulation, Chemical
  • Receptors, Adrenergic, beta
  • Propranolol
  • Isoproterenol
  • Erythrocytes
  • Erythrocyte Membrane
  • Enzyme Activation
  • Animals
  • Adenylyl Cyclases