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Thyroid hormone regulation of beta-adrenergic receptor number.

Publication ,  Journal Article
Williams, LT; Lefkowitz, RJ; Watanabe, AM; Hathaway, DR; Besch, HR
Published in: J Biol Chem
April 25, 1977

The effects of exogenous thyroid hormones (thyroxine and triiodothyronine) on beta-adrenergic receptors in the rat myocardium were investigated. The potent beta-adrenergic antagonist, (-)-[3H]dihydroalprenolol, was used to directly estimate the number and affinity of beta-adrenergic receptors in rat heart membranes from control and hyperthyroid rats. Cardiac membranes from hyperthyroid rats contained 196 +/- 7 fmol of (-)-[3H]dihydroalprenolol binding sites/mg of protein which was significantly (p less than 0.005) greater than the number of binding sites (89 +/- 5 fmol/mg of protein) present in control membranes. The equilibrium dissociation constant (KD) for the interaction of receptors with dihydroalprenolol was the same (2 to 15 nM) in membranes from control and hyperthyroid rats. Similarly, there was no significant difference between the control and hyperthyroid membranes in the affinity of the beta-adrenergic receptor binding sites for the beta-adrenergic agonist isoproterenol. The results of this study demonstrate that thyroid hormones can regulate the number of cardiac beta-adrenergic receptors. The increased numbers of receptors may be responsible, at least in part, for the enhanced catecholamine sensitivity of beta-adrenergic-coupled cardiac responses in the hyperthyroid state.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1977

Volume

252

Issue

8

Start / End Page

2787 / 2789

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Thyroxine
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Myocardium
  • Male
  • Kinetics
  • Isoproterenol
  • Hyperthyroidism
 

Citation

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Williams, L. T., Lefkowitz, R. J., Watanabe, A. M., Hathaway, D. R., & Besch, H. R. (1977). Thyroid hormone regulation of beta-adrenergic receptor number. J Biol Chem, 252(8), 2787–2789.
Williams, L. T., R. J. Lefkowitz, A. M. Watanabe, D. R. Hathaway, and H. R. Besch. “Thyroid hormone regulation of beta-adrenergic receptor number.J Biol Chem 252, no. 8 (April 25, 1977): 2787–89.
Williams LT, Lefkowitz RJ, Watanabe AM, Hathaway DR, Besch HR. Thyroid hormone regulation of beta-adrenergic receptor number. J Biol Chem. 1977 Apr 25;252(8):2787–9.
Williams, L. T., et al. “Thyroid hormone regulation of beta-adrenergic receptor number.J Biol Chem, vol. 252, no. 8, Apr. 1977, pp. 2787–89.
Williams LT, Lefkowitz RJ, Watanabe AM, Hathaway DR, Besch HR. Thyroid hormone regulation of beta-adrenergic receptor number. J Biol Chem. 1977 Apr 25;252(8):2787–2789.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1977

Volume

252

Issue

8

Start / End Page

2787 / 2789

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Thyroxine
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Myocardium
  • Male
  • Kinetics
  • Isoproterenol
  • Hyperthyroidism