Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.
Journal Article (Journal Article)
Incubation of purified frog erythrocyte membranes with beta-adrenergic agonists at 25 degrees produces relatively rapid (half-time about 10 min) desensitization (inactivation) of about 60% of the beta-adrenergic receptor binding sites. The desensitized receptors no longer bind the specific beta-adrenergic ligand (-)[3H]dihydroalprenolol. The decrease in the number of functional beta-adrenergic receptors is also manifest as a decreased ability of isoproterenol to stimulate the membrane-bound adenylate cyclase.
Full Text
Duke Authors
Cited Authors
- Mukherjee, C; Lefkowitz, RJ
Published Date
- May 1, 1976
Published In
Volume / Issue
- 73 / 5
Start / End Page
- 1494 - 1498
PubMed ID
- 5723
Pubmed Central ID
- PMC430323
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.73.5.1494
Language
- eng
Conference Location
- United States