Desensitization of beta-adrenergic receptors by beta-adrenergic agonists in a cell-free system: resensitization by guanosine 5'-(beta, gamma-imino)triphosphate and other purine nucleotides.

Journal Article (Journal Article)

Incubation of purified frog erythrocyte membranes with beta-adrenergic agonists at 25 degrees produces relatively rapid (half-time about 10 min) desensitization (inactivation) of about 60% of the beta-adrenergic receptor binding sites. The desensitized receptors no longer bind the specific beta-adrenergic ligand (-)[3H]dihydroalprenolol. The decrease in the number of functional beta-adrenergic receptors is also manifest as a decreased ability of isoproterenol to stimulate the membrane-bound adenylate cyclase.

Full Text

Duke Authors

Cited Authors

  • Mukherjee, C; Lefkowitz, RJ

Published Date

  • May 1, 1976

Published In

Volume / Issue

  • 73 / 5

Start / End Page

  • 1494 - 1498

PubMed ID

  • 5723

Pubmed Central ID

  • PMC430323

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.73.5.1494

Language

  • eng

Conference Location

  • United States