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[3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase

Publication ,  Journal Article
Lefkowitz, RJ
Published in: Biochemical and Biophysical Research Communications
1974

[3H]norepinephrine binds in vitro to microsomal membranes derived from a wide variety of tissues. Controversy exists as to the physiological significance of this binding phenomenon and recently the suggestion has been made that this represents binding to the enzyme catechol-o-methyl transferase (COMT). The enzyme and the [3H]norepinephrine binding sites however, are shown to have very different characteristics. Tissues having the highest levels of microsomal catechol-o-methyl transferase(liver, kidney) bind the least [3H]norepinephrine. The enzyme is concentrated in soluble fractions of tissue whereas the binding sites are found almost exclusively in membrane fractions. Purified preparations of the binding sites have no catechol-o-methyl transferase activity. pH optima, KM for epinephrine, and substrate specificity are all strikingly different for the enzyme and the [3H]norepinephrine binding sites. It is concluded that in vitro[3H]norepinephrine binding is unrelated to microsomal catechol-o-methyl transferase. © 1974 Academic Press, Inc.

Duke Scholars

Published In

Biochemical and Biophysical Research Communications

ISSN

0006-291X

Publication Date

1974

Volume

58

Issue

4

Start / End Page

1110 / 1118

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 1101 Medical Biochemistry and Metabolomics
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Chicago
ICMJE
MLA
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Lefkowitz, R. J. (1974). [3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase. Biochemical and Biophysical Research Communications, 58(4), 1110–1118.
Lefkowitz, R. J. “[3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase.” Biochemical and Biophysical Research Communications 58, no. 4 (1974): 1110–18.
Lefkowitz RJ. [3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase. Biochemical and Biophysical Research Communications. 1974;58(4):1110–8.
Lefkowitz, R. J. “[3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase.” Biochemical and Biophysical Research Communications, vol. 58, no. 4, 1974, pp. 1110–18.
Lefkowitz RJ. [3H]Norepinephrine binding: Unrelated to catechol-o-methyl transferase. Biochemical and Biophysical Research Communications. 1974;58(4):1110–1118.
Journal cover image

Published In

Biochemical and Biophysical Research Communications

ISSN

0006-291X

Publication Date

1974

Volume

58

Issue

4

Start / End Page

1110 / 1118

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 1101 Medical Biochemistry and Metabolomics
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry