Protein kinase C zeta is associated with the mitotic apparatus in primary cell cultures of the shark rectal gland.


Journal Article

Protein kinase C zeta (PKC zeta) is an atypical PKC isoform that has recently been implicated in cell division and cell growth. However, there has been no morphologic evidence for the involvement of PKC zeta in mitogenic signal transduction. Here we use immunocytochemistry to demonstrate that PKC zeta co-localizes with microtubules in both interphase and metaphase cells of the shark rectal gland in primary culture. This co-localization is present after non-ionic detergent treatment and is disrupted by nocodazole. During mitosis, PKC zeta is associated with the mitotic apparatus and co-localizes with beta-tubulin in spindle microtubules, while entirely sparing astral microtubules. These findings provide the first evidence that PKC zeta is associated with the mitotic apparatus. The striking presence of PKC zeta in the central portion of the mitotic apparatus suggests a functional role for this kinase isoform in cell division.

Full Text

Duke Authors

Cited Authors

  • Lehrich, RW; Forrest, JN

Published Date

  • December 23, 1994

Published In

Volume / Issue

  • 269 / 51

Start / End Page

  • 32446 - 32450

PubMed ID

  • 7798244

Pubmed Central ID

  • 7798244

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States