Effects of topographical and mechanical property alterations induced by oxygen plasma modification on stem cell behavior.

Polymeric substrates intended for cell culture and tissue engineering are often surface-modified to facilitate cell attachment of most anchorage-dependent cell types. The modification alters the surface chemistry and possibly topography. However, scant attention has been paid to other surface property alterations. In studying oxygen plasma treatment of polydimethylsiloxane (PDMS), we show that oxygen plasma treatment alters the surface chemistry and, consequently, the topography and elasticity of PDMS at the nanoscale level. The elasticity factor has the predominant effect, compared with the chemical and topographical factors, on cell adhesions of human mesenchymal stem cells (hMSCs). The enhanced focal adhesions favor cell spreading and osteogenesis of hMSCs. Given the prevalent use of PDMS in biomedical device construction and cell culture experiments, this study highlights the importance of understanding how oxygen plasma treatment would impact subsequent cell-substrate interactions. It helps explain inconsistency in the literature and guides preparation of PDMS-based biomedical devices in the future.

Full Text

Duke Authors

Cited Authors

  • Yang, Y; Kulangara, K; Lam, RT; Dharmawan, R; Leong, KW

Published Date

  • October 23, 2012

Published In

Volume / Issue

  • 6 / 10

Start / End Page

  • 8591 - 8598

PubMed ID

  • 22970773

Electronic International Standard Serial Number (EISSN)

  • 1936-086X

Digital Object Identifier (DOI)

  • 10.1021/nn301713d

Language

  • eng