Stem cell differentiation indicated by noninvasive photonic characterization and fractal analysis of subcellular architecture.

Journal Article

We hypothesised that global structural changes in stem cells would manifest with differentiation, and that these changes would be observable with light scattering microscopy. Analysed with a fractal dimension formalism, we observed significant structural changes in differentiating human mesenchymal stem cells within one day after induction, earlier than could be detected by gene expression profiling. Moreover, light scattering microscopy is entirely non-perturbative, so the same sample could be monitored throughout the differentiation process. We explored one possible mechanism, chromatin remodelling, to account for the changes we observed. Correlating with the staining of HP1α, a heterochromatin protein, we applied novel microscopy methods and fractal analysis to monitor the plastic dynamics of chromatin within stem cell nuclei. We showed that the level of chromatin condensation changed during differentiation, and provide one possible explanation for the changes seen with the light scattering method. These results lend physical insight into stem cell differentiation while providing physics-based methods for non-invasive detection of the differentiation process.

Full Text

Duke Authors

Cited Authors

  • Chalut, KJ; Kulangara, K; Wax, A; Leong, KW

Published Date

  • August 2011

Published In

Volume / Issue

  • 3 / 8

Start / End Page

  • 863 - 867

PubMed ID

  • 21695342

Electronic International Standard Serial Number (EISSN)

  • 1757-9708

Digital Object Identifier (DOI)

  • 10.1039/c1ib00003a

Language

  • eng

Conference Location

  • England