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Efficacy of engineered FVIII-producing skeletal muscle enhanced by growth factor-releasing co-axial electrospun fibers.

Publication ,  Journal Article
Liao, I-C; Leong, KW
Published in: Biomaterials
February 2011

Co-axial electrospun fibers can offer both topographical and biochemical cues for tissue engineering applications. In this study, we demonstrate the sustained treatment of hemophilia through a non-viral, tissue engineering approach facilitated by growth factor-releasing co-axial electrospun fibers. FVIII-producing skeletal myotubes were first engineered on aligned electrospun fibers in vitro, followed by implantation in hemophilic mice with or without a layer of core-shell electrospun fibers designed to provide sustained delivery of angiogenic or lymphangiogenic growth factors, which serves to stimulate the lymphatic or vascular systems to enhance the FVIII transport from the implant site into systemic circulation. Upon subcutaneous implantation into hemophilic mice, the construct seamlessly integrated with the host tissue within one month, and specifically induced either vascular or lymphatic network infiltration in accordance with the growth factors released from the electrospun fibers. Engineered constructs that induced angiogenesis resulted in sustained elevation of plasma FVIII and significantly reduced blood coagulation time for at least 2-months. Biomaterials-assisted functional tissue engineering was shown in this study to offer protein replacement therapy for a genetic disorder such as hemophilia.

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

February 2011

Volume

32

Issue

6

Start / End Page

1669 / 1677

Related Subject Headings

  • Tissue Engineering
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Mice
  • Humans
  • Hemophilia A
  • Factor VIII
  • Cells, Cultured
  • Biomedical Engineering
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liao, I.-C., & Leong, K. W. (2011). Efficacy of engineered FVIII-producing skeletal muscle enhanced by growth factor-releasing co-axial electrospun fibers. Biomaterials, 32(6), 1669–1677. https://doi.org/10.1016/j.biomaterials.2010.10.049
Liao, I-Chien, and Kam W. Leong. “Efficacy of engineered FVIII-producing skeletal muscle enhanced by growth factor-releasing co-axial electrospun fibers.Biomaterials 32, no. 6 (February 2011): 1669–77. https://doi.org/10.1016/j.biomaterials.2010.10.049.
Liao, I. Chien, and Kam W. Leong. “Efficacy of engineered FVIII-producing skeletal muscle enhanced by growth factor-releasing co-axial electrospun fibers.Biomaterials, vol. 32, no. 6, Feb. 2011, pp. 1669–77. Epmc, doi:10.1016/j.biomaterials.2010.10.049.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

February 2011

Volume

32

Issue

6

Start / End Page

1669 / 1677

Related Subject Headings

  • Tissue Engineering
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Mice
  • Humans
  • Hemophilia A
  • Factor VIII
  • Cells, Cultured
  • Biomedical Engineering
  • Animals