Ex vivo expansion of hematopoietic primitive cells on ECM molecule-immobilized scaffold
The ex vivo expansion of hematopoietic stem sells (HSC) on extracellular matrix (ECM) molecule-immobilized scaffold was investigated. The scaffold used was a fibronectin and collagen conjugated polyethylene terephthalate (PET) scaffold. Bone marrow CD34+ cells were cultured in the modified PET scaffolds in a 96-well plate using serum free StemSpam medium supplemented with cytokines. It was observed that the scaffolds surface-conjugated with ECM molecules served as an effective substrate for the expansion of hematopoietic primitive cells. Fibronectin conjugated scaffold was found to exhibit the highest extent of CD34+ cell expansion.