Differential acetylcholinesterase inhibition of chlorpyrifos, diazinon and parathion in larval zebrafish.

Journal Article (Journal Article)

Zebrafish are increasingly used for developmental neurotoxicity testing because early embryonic events are easy to visualize, exposures are done without affecting the mother and the rapid development of zebrafish allows for high throughput testing. We used zebrafish to examine how exposures to three different organophosphorus pesticides (chlorpyrifos, diazinon and parathion) over the first five days of embryonic and larval development of zebrafish affected their survival, acetylcholinesterase (AChE) activity and behavior. We show that at non-lethal, equimolar concentrations, chlorpyrifos (CPF) is more effective at equimolar concentrations than diazinon (DZN) and parathion (PA) in producing AChE inhibition. As concentrations of DZN and PA are raised, lethality occurs before they can produce the degree of AChE inhibition observed with CPF at 300 nM. Because of its availability outside the mother at the time of fertilization, zebrafish provides a complementary model for studying the neurotoxicity of very early developmental exposures.

Full Text

Duke Authors

Cited Authors

  • Yen, J; Donerly, S; Levin, ED; Linney, EA

Published Date

  • November 2011

Published In

Volume / Issue

  • 33 / 6

Start / End Page

  • 735 - 741

PubMed ID

  • 22036888

Pubmed Central ID

  • PMC3225599

Electronic International Standard Serial Number (EISSN)

  • 1872-9738

Digital Object Identifier (DOI)

  • 10.1016/j.ntt.2011.10.004


  • eng

Conference Location

  • United States