Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety.

Published

Journal Article

Zebrafish are becoming more widely used to study neurobehavioral pharmacology. We have developed a method to assess novel environment diving behavior of zebrafish as a model of stress response and anxiolytic drug effects. In a novel tank, zebrafish dwell in the bottom of the tank initially and then increase their swimming exploration to higher levels over time. We previously found that nicotine, which has anxiolytic effects in rodents and humans, significantly lessens the novel tank diving response in zebrafish. The specificity of the diving effect was validated with a novel vs. non-novel test tank. The novel tank diving response of zebrafish was tested when given three anxiolytic drugs from two different chemical and pharmacological classes: buspirone, chlordiazepoxide and diazepam. When the test tank was novel the diving response was clearly seen whereas it was significantly reduced when the test tank was not novel. Buspirone, a serotonergic (5HT(1A) receptor agonist) anxiolytic drug with some D(2) dopaminergic effect, had a pronounced anxiolytic-like effect in the zebrafish diving model at doses that did not have sedative effects. In contrast, chlordiazepoxide, a benzodiazepine anxiolytic drug, which is an effective agonist at GABA-A receptors, did not produce signs of anxiolysis in zebrafish over a broad dose range up to those that caused sedation. Diazepam another benzodiazepine anxiolytic drug did produce an anxiolytic effect at doses that did not cause sedation. The zebrafish novel tank diving task can be useful in discriminating anxiolytic drugs of several classes (serotonergic, benzodiazepines and nicotinic).

Full Text

Duke Authors

Cited Authors

  • Bencan, Z; Sledge, D; Levin, ED

Published Date

  • November 2009

Published In

Volume / Issue

  • 94 / 1

Start / End Page

  • 75 - 80

PubMed ID

  • 19643124

Pubmed Central ID

  • 19643124

Electronic International Standard Serial Number (EISSN)

  • 1873-5177

Digital Object Identifier (DOI)

  • 10.1016/j.pbb.2009.07.009

Language

  • eng

Conference Location

  • United States