Transcriptional profiling of whole blood and serum protein analysis of mice exposed to the neurotoxin Pacific Ciguatoxin-1.

Published

Journal Article

Ciguatoxins (CTX) are a suite of cyclic polyether toxins produced by the marine dinoflagellate Gambierdiscus sp., are potent activators of voltage-gated sodium channels and a leading cause of human poisoning from food fish. This report characterizes the genomic and proteomic response in whole blood of adult male mice exposed i.p. to 264 ng/kg of the Pacific congener of CTX (P-CTX-1) at 1, 4 and 24h. Whole genome microarray expression data were filtered by tightness of fit between replicates, fold change (1.8) and p-value (10(-5)), resulting in 183 annotated genes used for trending analysis, K-means clustering and ontology classification. Genes involved with cytokine signaling, proteasome complex and ribosomal function were dominant. qPCR performed on 19 genes of interest had a correlation of 0.95 to array results by Pearson's correlation coefficient. Serum protein analysis showed small but significant changes in 6 of 60 proteins assayed: Ccl2, Ccl12, CD40, IL-10, leptin and M-CSF. In large part, the gene expression was consistent with a Th2 immune response with interesting similarities to expression seen in asthmatic models.

Full Text

Duke Authors

Cited Authors

  • Ryan, JC; Bottein Dechraoui, M-Y; Morey, JS; Rezvani, A; Levin, ED; Gordon, CJ; Ramsdell, JS; Van Dolah, FM

Published Date

  • November 2007

Published In

Volume / Issue

  • 28 / 6

Start / End Page

  • 1099 - 1109

PubMed ID

  • 17868886

Pubmed Central ID

  • 17868886

International Standard Serial Number (ISSN)

  • 0161-813X

Digital Object Identifier (DOI)

  • 10.1016/j.neuro.2007.05.013

Language

  • eng

Conference Location

  • Netherlands