Transcriptional profiling of whole blood and serum protein analysis of mice exposed to the neurotoxin Pacific Ciguatoxin-1.
Journal Article (Journal Article)
Ciguatoxins (CTX) are a suite of cyclic polyether toxins produced by the marine dinoflagellate Gambierdiscus sp., are potent activators of voltage-gated sodium channels and a leading cause of human poisoning from food fish. This report characterizes the genomic and proteomic response in whole blood of adult male mice exposed i.p. to 264 ng/kg of the Pacific congener of CTX (P-CTX-1) at 1, 4 and 24h. Whole genome microarray expression data were filtered by tightness of fit between replicates, fold change (1.8) and p-value (10(-5)), resulting in 183 annotated genes used for trending analysis, K-means clustering and ontology classification. Genes involved with cytokine signaling, proteasome complex and ribosomal function were dominant. qPCR performed on 19 genes of interest had a correlation of 0.95 to array results by Pearson's correlation coefficient. Serum protein analysis showed small but significant changes in 6 of 60 proteins assayed: Ccl2, Ccl12, CD40, IL-10, leptin and M-CSF. In large part, the gene expression was consistent with a Th2 immune response with interesting similarities to expression seen in asthmatic models.
Full Text
Duke Authors
Cited Authors
- Ryan, JC; Bottein Dechraoui, M-Y; Morey, JS; Rezvani, A; Levin, ED; Gordon, CJ; Ramsdell, JS; Van Dolah, FM
Published Date
- November 2007
Published In
Volume / Issue
- 28 / 6
Start / End Page
- 1099 - 1109
PubMed ID
- 17868886
International Standard Serial Number (ISSN)
- 0161-813X
Digital Object Identifier (DOI)
- 10.1016/j.neuro.2007.05.013
Language
- eng
Conference Location
- Netherlands