Apoptotic caspases regulate induction of iPSCs from human fibroblasts.

Published

Journal Article

The molecular mechanisms involved in the derivation of induced pluripotent stem cells (iPSCs) from differentiated cells are poorly understood. Here we report that caspases 3 and 8, two proteases associated with apoptotic cell death, play critical roles in induction of iPSCs from human fibroblasts. Activation of caspases 3 and 8 occurs soon after transduction of iPSC-inducing transcription factors. Oct-4, a key iPSC transcription factor, is responsible for the activation. Inhibition of caspase 3 or 8 in human fibroblast cells partially or completely (respectively) prevents the induction of iPSCs. Furthermore, retinoblastoma susceptibility (Rb) protein appears to be one of the factors that act downstream of the caspases. We propose that caspases are key facilitators of nuclear reprogramming in iPSC induction.

Full Text

Duke Authors

Cited Authors

  • Li, F; He, Z; Shen, J; Huang, Q; Li, W; Liu, X; He, Y; Wolf, F; Li, C-Y

Published Date

  • October 8, 2010

Published In

Volume / Issue

  • 7 / 4

Start / End Page

  • 508 - 520

PubMed ID

  • 20887956

Pubmed Central ID

  • 20887956

Electronic International Standard Serial Number (EISSN)

  • 1875-9777

Digital Object Identifier (DOI)

  • 10.1016/j.stem.2010.09.003

Language

  • eng

Conference Location

  • United States