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Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging.

Publication ,  Journal Article
Ji, X; Cheng, L; Wei, F; Li, H; Wang, M; Tian, Y; Chen, X; Wang, Y; Wolf, F; Li, C; Huang, Q
Published in: Invest Ophthalmol Vis Sci
December 2009

PURPOSE: To establish human retinoblastoma (RB) animal models that allow sensitive, noninvasive and continuous monitoring of tumor growth and metastasis in vivo. METHODS: The human RB tumor cell lines HXO-Rb44 and Y79 were engineered to express a fusion reporter gene allowing for bioluminescence and fluorescence imaging. Intraocular and metastatic tumors were induced in immunodeficient nude mice by injection of bioluminescent RB cells into eye compartments and into the left ventricle or tail vein. The growth kinetics of intraocular and metastatic tumors was quantitatively and continuously monitored via bioluminescence imaging (BLI). RESULTS: Intraocular injection of HXO-Rb44-GFP-luc cells resulted in 100%, 80%, and 80% successful RB tumor development in the anterior chamber, vitreal cavity and subretinal space, respectively. The subretinal injection of Y79-GFP-luc cells resulted in 100% tumor development. BLI signal intensity correlated with the number of tumor cells injected as well as the weight of the tumor-bearing eyes. After bilateral subretinal injection of HXO-Rb44-GFP-luc cells, one of six RB tumor mice developed brain metastasis. Intracardiac injection of HXO-Rb44-GFP-luc cells resulted in metastatic disease in 9 of 15 nude mice, whereas tail vein injection resulted in metastasis in 1 of 16. Metastases were developed in multiple organs, including lymph nodes, bone, and brain, resembling the metastatic profile in patients with RB. CONCLUSIONS: BLI allowed sensitive, noninvasive, and quantitative localization and monitoring of intraocular and metastatic RB tumor growth in vivo and thus may be a useful tool to study RB biology as well as anti-RB therapies.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

December 2009

Volume

50

Issue

12

Start / End Page

5544 / 5551

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Retinoblastoma
  • Retinal Neoplasms
  • Recombinant Fusion Proteins
  • Ophthalmology & Optometry
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ji, X., Cheng, L., Wei, F., Li, H., Wang, M., Tian, Y., … Huang, Q. (2009). Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging. Invest Ophthalmol Vis Sci, 50(12), 5544–5551. https://doi.org/10.1167/iovs.08-3258
Ji, Xunda, Lin Cheng, Fang Wei, Huiming Li, Mengyun Wang, Yuhua Tian, Xiafang Chen, et al. “Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging.Invest Ophthalmol Vis Sci 50, no. 12 (December 2009): 5544–51. https://doi.org/10.1167/iovs.08-3258.
Ji X, Cheng L, Wei F, Li H, Wang M, Tian Y, et al. Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5544–51.
Ji, Xunda, et al. “Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging.Invest Ophthalmol Vis Sci, vol. 50, no. 12, Dec. 2009, pp. 5544–51. Pubmed, doi:10.1167/iovs.08-3258.
Ji X, Cheng L, Wei F, Li H, Wang M, Tian Y, Chen X, Wang Y, Wolf F, Li C, Huang Q. Noninvasive visualization of retinoblastoma growth and metastasis via bioluminescence imaging. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):5544–5551.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

December 2009

Volume

50

Issue

12

Start / End Page

5544 / 5551

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Retinoblastoma
  • Retinal Neoplasms
  • Recombinant Fusion Proteins
  • Ophthalmology & Optometry
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice, Inbred BALB C
  • Mice