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High intensity focused ultrasound induced gene activation in solid tumors

Publication ,  Journal Article
Liu, Y; Kon, T; Li, C; Zhong, P
Published in: AIP Conference Proceedings
May 8, 2006

In this work, the feasibility of using high intensity focused ultrasound (HIFU) to activate trans-gene expression in a mouse tumor model was investigated. 4T1 cancer cells were implanted subcutaneously in the hind limbs of Balb/C mice and adenovirus luciferase gene vectors under the control of heat shock protein 70B promoter (Adeno-hsp70B-Luc) were injected intratumoraly for gene transfection. One day following the virus injection, the transfected tumors were heated to a peak temperature of 55, 65, 75, and 85°C, respectively, in 10s at multiple sites around the center of the tumor using a HIFU transducer operated at either 1.1-MHz (fundamental) or 3.3-MHz (3 rd harmonic) frequency. Inducible luciferase gene expression was found to vary from 15-fold to 120-fold of the control group following 1.1-MHz HIFU exposure. The maximum gene activation was produced at a peak temperature of 65∼75°C one day following HIFU exposure and decayed gradually to baseline level within 7 days. The inducible gene activation produced by 3.3-MHz HIFU exposure (75°C-10s) was found to be comparable to that produced by hyperthermia (42°C-30min). Altogether, these results demonstrate the feasibility of using HIFU as a simple and versatile physical means to regulate trans-gene expression in vivo. This unique feature may be explored in the future for a synergistic combination of HIFU-induced thermal ablation with heat-induced gene therapy for improved cancer therapy. © 2006 American Institute of Physics.

Duke Scholars

Published In

AIP Conference Proceedings

DOI

EISSN

1551-7616

ISSN

0094-243X

Publication Date

May 8, 2006

Volume

829

Start / End Page

588 / 592
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liu, Y., Kon, T., Li, C., & Zhong, P. (2006). High intensity focused ultrasound induced gene activation in solid tumors. AIP Conference Proceedings, 829, 588–592. https://doi.org/10.1063/1.2205542
Liu, Y., T. Kon, C. Li, and P. Zhong. “High intensity focused ultrasound induced gene activation in solid tumors.” AIP Conference Proceedings 829 (May 8, 2006): 588–92. https://doi.org/10.1063/1.2205542.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound induced gene activation in solid tumors. AIP Conference Proceedings. 2006 May 8;829:588–92.
Liu, Y., et al. “High intensity focused ultrasound induced gene activation in solid tumors.” AIP Conference Proceedings, vol. 829, May 2006, pp. 588–92. Scopus, doi:10.1063/1.2205542.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound induced gene activation in solid tumors. AIP Conference Proceedings. 2006 May 8;829:588–592.

Published In

AIP Conference Proceedings

DOI

EISSN

1551-7616

ISSN

0094-243X

Publication Date

May 8, 2006

Volume

829

Start / End Page

588 / 592