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Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results.

Publication ,  Journal Article
Plathow, C; Lohr, F; Divkovic, G; Rademaker, G; Farhan, N; Peschke, P; Zuna, I; Debus, J; Claussen, CD; Kauczor, H-U; Li, CY; Jenne, J; Huber, P
Published in: Invest Radiol
November 2005

OBJECTIVE: We sought to examine high-intensity focused ultrasound (HIFU)-induced hyperthermia in the liver of a rat model to focally induce green-fluorescent protein (GFP). MATERIALS AND METHODS: A total of 25 Copenhagen rats were included in this study. Rats were divided into groups treated with an adenovirus coding for green fluorescent protein (GFP) under the control of a hsp70B promoter and a CMV promoter. Ad-CMV-GFP-treated rats served as positive control. Untreated controls only subjected to MRI +/- HIFU-treatment served to find out optimal power of HIFU in the target area of the liver. Temperature was noninvasively monitored by temperature sensitive magnetic resonance imaging (MRI). RESULTS: Rats treated with Ad-hsp70B-GFP demonstrated localized gene induction within the liver parenchyma, in good correlation with MRI and histology. Applying an acoustic power of 1.92 W a relatively uniform focal temperature up to 42 +/- 5 degrees C within the liver parenchyma could be documented. 3 x 10(9) plaque-forming units proved to account for a very homogeneous liver infection. Number of fluorescent cells in the region of hyperthermia was similar to the control group treated with Ad-CMV-GFP. CONCLUSION: Using the introduced parameters spatially controlled gene induction within a parenchymal organ such as the liver in rats using HIFU under control of MRI is feasible.

Duke Scholars

Published In

Invest Radiol

DOI

ISSN

0020-9996

Publication Date

November 2005

Volume

40

Issue

11

Start / End Page

729 / 735

Location

United States

Related Subject Headings

  • Ultrasonography
  • Transgenes
  • Transcriptional Activation
  • Rats, Inbred Strains
  • Rats
  • Promoter Regions, Genetic
  • Nuclear Medicine & Medical Imaging
  • Models, Animal
  • Microscopy, Fluorescence
  • Male
 

Citation

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ICMJE
MLA
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Plathow, C., Lohr, F., Divkovic, G., Rademaker, G., Farhan, N., Peschke, P., … Huber, P. (2005). Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results. Invest Radiol, 40(11), 729–735. https://doi.org/10.1097/01.rli.0000184763.62578.06
Plathow, Christian, Frank Lohr, Gabriela Divkovic, Guido Rademaker, Nabeel Farhan, Peter Peschke, Ivan Zuna, et al. “Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results.Invest Radiol 40, no. 11 (November 2005): 729–35. https://doi.org/10.1097/01.rli.0000184763.62578.06.
Plathow C, Lohr F, Divkovic G, Rademaker G, Farhan N, Peschke P, et al. Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results. Invest Radiol. 2005 Nov;40(11):729–35.
Plathow, Christian, et al. “Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results.Invest Radiol, vol. 40, no. 11, Nov. 2005, pp. 729–35. Pubmed, doi:10.1097/01.rli.0000184763.62578.06.
Plathow C, Lohr F, Divkovic G, Rademaker G, Farhan N, Peschke P, Zuna I, Debus J, Claussen CD, Kauczor H-U, Li CY, Jenne J, Huber P. Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results. Invest Radiol. 2005 Nov;40(11):729–735.

Published In

Invest Radiol

DOI

ISSN

0020-9996

Publication Date

November 2005

Volume

40

Issue

11

Start / End Page

729 / 735

Location

United States

Related Subject Headings

  • Ultrasonography
  • Transgenes
  • Transcriptional Activation
  • Rats, Inbred Strains
  • Rats
  • Promoter Regions, Genetic
  • Nuclear Medicine & Medical Imaging
  • Models, Animal
  • Microscopy, Fluorescence
  • Male