Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results.

Published

Journal Article

OBJECTIVE: We sought to examine high-intensity focused ultrasound (HIFU)-induced hyperthermia in the liver of a rat model to focally induce green-fluorescent protein (GFP). MATERIALS AND METHODS: A total of 25 Copenhagen rats were included in this study. Rats were divided into groups treated with an adenovirus coding for green fluorescent protein (GFP) under the control of a hsp70B promoter and a CMV promoter. Ad-CMV-GFP-treated rats served as positive control. Untreated controls only subjected to MRI +/- HIFU-treatment served to find out optimal power of HIFU in the target area of the liver. Temperature was noninvasively monitored by temperature sensitive magnetic resonance imaging (MRI). RESULTS: Rats treated with Ad-hsp70B-GFP demonstrated localized gene induction within the liver parenchyma, in good correlation with MRI and histology. Applying an acoustic power of 1.92 W a relatively uniform focal temperature up to 42 +/- 5 degrees C within the liver parenchyma could be documented. 3 x 10(9) plaque-forming units proved to account for a very homogeneous liver infection. Number of fluorescent cells in the region of hyperthermia was similar to the control group treated with Ad-CMV-GFP. CONCLUSION: Using the introduced parameters spatially controlled gene induction within a parenchymal organ such as the liver in rats using HIFU under control of MRI is feasible.

Full Text

Duke Authors

Cited Authors

  • Plathow, C; Lohr, F; Divkovic, G; Rademaker, G; Farhan, N; Peschke, P; Zuna, I; Debus, J; Claussen, CD; Kauczor, H-U; Li, CY; Jenne, J; Huber, P

Published Date

  • November 2005

Published In

Volume / Issue

  • 40 / 11

Start / End Page

  • 729 - 735

PubMed ID

  • 16230906

Pubmed Central ID

  • 16230906

International Standard Serial Number (ISSN)

  • 0020-9996

Digital Object Identifier (DOI)

  • 10.1097/01.rli.0000184763.62578.06

Language

  • eng

Conference Location

  • United States