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[Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway].

Publication ,  Journal Article
Wang, F; Tian, Y-H; Li, L; Chen, X-F; Hu, H-H; Li, C-Y; Huang, Q
Published in: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
March 2002

Solid tumors require an adequate vascular supply to grow beyond a certain dimension. It is known that formation of new blood vessels in tumor is mediated by unbalanced expression of angiogenic factors and their inhibitors. Among the former, the vascular endothelial growth factor (VEGF) has been assumed prime candidacy as a major positive physiological effector. To investigate the role of VEGF in angiogenesis associated with development of breast cancer, a sense VEGF and an anti-sense VEGF expression plasmids were constructed, and then were introduced into a human breast carcinoma cell line, MCF-7, expressing middle level of endogenous VEGF. Anti-sense VEGF(121) transfected MCF-7 cells that expressed reduced constitutive levels of VEGF and showed the same growing potential as untransfected MCF-7 cells in vitro, but it showed longer latency, smaller tumor, slower growth and prolonged survival time compared to parental or sense VEGF(165) transfected MCF-7 cells in vivo. Moreover, the tumors derived from anti-sense VEGF(121) transfected MCF-7 cells characterized by minimal vascularization and extensive necrosis. Finally, mice with primary subcutaneous tumors treated with intratumoral administration of anti-sense VEGF, or the plasmid expressing extracellular domain of the Flk-1 VEGF receptor (sFlk-1) followed by electroporation, showed significant tumor suppression. These results suggest that VEGF plays a major angiogenic role in breast cancer and a strategy, which blocks the VEGF paracrine pathway, may provide a means to control tumor growth topically without the risk of systemic antiangiogenesis.

Duke Scholars

Published In

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

ISSN

0582-9879

Publication Date

March 2002

Volume

34

Issue

2

Start / End Page

165 / 170

Location

China

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Transfection
  • Signal Transduction
  • Neovascularization, Pathologic
  • Neoplasm Transplantation
  • Neoplasm Metastasis
  • Mice, Nude
 

Citation

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MLA
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Wang, F., Tian, Y.-H., Li, L., Chen, X.-F., Hu, H.-H., Li, C.-Y., & Huang, Q. (2002). [Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), 34(2), 165–170.
Wang, Feng, Yu-Hua Tian, Li Li, Xia-Fang Chen, Hong-Hui Hu, Chuan-Yuan Li, and Qian Huang. “[Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway].Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34, no. 2 (March 2002): 165–70.
Wang F, Tian Y-H, Li L, Chen X-F, Hu H-H, Li C-Y, et al. [Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Mar;34(2):165–70.
Wang, Feng, et al. “[Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway].Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), vol. 34, no. 2, Mar. 2002, pp. 165–70.
Wang F, Tian Y-H, Li L, Chen X-F, Hu H-H, Li C-Y, Huang Q. [Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Mar;34(2):165–170.

Published In

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)

ISSN

0582-9879

Publication Date

March 2002

Volume

34

Issue

2

Start / End Page

165 / 170

Location

China

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Transfection
  • Signal Transduction
  • Neovascularization, Pathologic
  • Neoplasm Transplantation
  • Neoplasm Metastasis
  • Mice, Nude