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[Using Hsp70 promoter to regulate target gene expression in tumor].

Publication ,  Journal Article
Huang, Q; Li, C
Published in: Zhonghua Bing Li Xue Za Zhi
June 2001

OBJECTIVE: To regulate gene expression in the desired tumor cells both in vitro and in vivo. METHODS: Heat inducible green fluorescent protein (GFP) expression plasmid and adenovirus were built by using different sizes of 5' end regulating sequence from human heat shock protein (Hsp) gene as promoter and GFP cDNA as report gene. GFP expression turned on by heating was observed in the cultured cells and the tumor grown in the dorsal skin window chamber. RESULTS: A 400 bp of Hsp gene 5' end regulating sequence can be activated by heating and it can drive report gene expression effectively both in vitro and in vivo. CONCLUSIONS: Heating can selectively induce gene expression in the targeted tumor. It provides an useful tool for cancer gene therapy because it possibly maximizes tumor cell killing and minimizes normal tissue damage.

Duke Scholars

Published In

Zhonghua Bing Li Xue Za Zhi

ISSN

0529-5807

Publication Date

June 2001

Volume

30

Issue

3

Start / End Page

198 / 201

Location

China

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • Promoter Regions, Genetic
  • Pathology
  • Mammary Neoplasms, Experimental
  • Hot Temperature
  • HSP70 Heat-Shock Proteins
  • Green Fluorescent Proteins
  • Genes, Reporter
  • Gene Expression Regulation
 

Citation

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Huang, Q., & Li, C. (2001). [Using Hsp70 promoter to regulate target gene expression in tumor]. Zhonghua Bing Li Xue Za Zhi, 30(3), 198–201.
Huang, Q., and C. Li. “[Using Hsp70 promoter to regulate target gene expression in tumor].Zhonghua Bing Li Xue Za Zhi 30, no. 3 (June 2001): 198–201.
Huang Q, Li C. [Using Hsp70 promoter to regulate target gene expression in tumor]. Zhonghua Bing Li Xue Za Zhi. 2001 Jun;30(3):198–201.
Huang, Q., and C. Li. “[Using Hsp70 promoter to regulate target gene expression in tumor].Zhonghua Bing Li Xue Za Zhi, vol. 30, no. 3, June 2001, pp. 198–201.
Huang Q, Li C. [Using Hsp70 promoter to regulate target gene expression in tumor]. Zhonghua Bing Li Xue Za Zhi. 2001 Jun;30(3):198–201.

Published In

Zhonghua Bing Li Xue Za Zhi

ISSN

0529-5807

Publication Date

June 2001

Volume

30

Issue

3

Start / End Page

198 / 201

Location

China

Related Subject Headings

  • Rats, Inbred F344
  • Rats
  • Promoter Regions, Genetic
  • Pathology
  • Mammary Neoplasms, Experimental
  • Hot Temperature
  • HSP70 Heat-Shock Proteins
  • Green Fluorescent Proteins
  • Genes, Reporter
  • Gene Expression Regulation