Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach.

Published

Journal Article

An efficient approach to the large-scale production of rAAV will significantly facilitate the application of this promising gene delivery vector in human gene therapy applications. In this study, we report a novel approach to rAAV production that is based exclusively on the adenovirus vector, without the need for plasmid transfections and special packaging cell lines. All components required for rAAV production, including the rep and cap genes, and the therapeutic gene(s) are delivered to the widely used 293 packaging cell line by adenovirus vectors. High-titer rAAV vectors (200-600 infectious units/producer cell) were obtained by use of this approach. As adenovirus vectors can be produced to high titers and they can infect cells in suspension efficiently, this approach may be amenable to scaled-up production of rAAV vectors.

Full Text

Duke Authors

Cited Authors

  • Zhang, X; Li, CY

Published Date

  • May 2001

Published In

Volume / Issue

  • 3 / 5 Pt 1

Start / End Page

  • 787 - 792

PubMed ID

  • 11356083

Pubmed Central ID

  • 11356083

International Standard Serial Number (ISSN)

  • 1525-0016

Digital Object Identifier (DOI)

  • 10.1006/mthe.2001.0306

Language

  • eng

Conference Location

  • United States