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Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation.

Publication ,  Journal Article
Huang, H; Li, CY; Little, JB
Published in: Int J Radiat Biol
August 1996

Suppression of wild-type p53 expression has been shown to enhance the radiation resistance of human diploid fibroblasts, but results concerning the role of p53 expression in the sensitivity of human tumour cells have been conflicting. In order to address this question, we transfected four human tumour cell lines with the human papilloma virus 16 E6 gene and compared the radiosensitivity of subclones expressing E6 with that of subclones transfected with the neo gene alone. E6 binds to wild-type p53 promoting its degradation and abrogating its function. Two of these cell lines, one derived from a squamous cell carcinoma and the other an osteogenic sarcoma, expressed wild-type p53. The other two cell lines were of similar origins and histologies but expressed mutant or no p53 (null). Insertion of E6 into the cell was accomplished by two techniques: (1) to-transfection of plasmid vectors containing neo and E6; (2) infection with a retroviral vector containing neo and E6. Multiple transfected subclones were examined for each cell line. Transfection with E6 and abrogation of p53 function had no significant influence on the radiosensitivity of any of the cell lines tested. In particular, there was no evidence that loss of wild-type p53 function increased the resistance of these human tumour cell lines to ionizing radiation.

Duke Scholars

Published In

Int J Radiat Biol

DOI

ISSN

0955-3002

Publication Date

August 1996

Volume

70

Issue

2

Start / End Page

151 / 160

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Repressor Proteins
  • Radiation Tolerance
  • Papillomaviridae
  • Oncology & Carcinogenesis
  • Oncogene Proteins, Viral
  • Neoplasms
  • Molecular Sequence Data
  • Humans
 

Citation

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Huang, H., Li, C. Y., & Little, J. B. (1996). Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation. Int J Radiat Biol, 70(2), 151–160. https://doi.org/10.1080/095530096145148
Huang, H., C. Y. Li, and J. B. Little. “Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation.Int J Radiat Biol 70, no. 2 (August 1996): 151–60. https://doi.org/10.1080/095530096145148.
Huang, H., et al. “Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation.Int J Radiat Biol, vol. 70, no. 2, Aug. 1996, pp. 151–60. Pubmed, doi:10.1080/095530096145148.
Journal cover image

Published In

Int J Radiat Biol

DOI

ISSN

0955-3002

Publication Date

August 1996

Volume

70

Issue

2

Start / End Page

151 / 160

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Repressor Proteins
  • Radiation Tolerance
  • Papillomaviridae
  • Oncology & Carcinogenesis
  • Oncogene Proteins, Viral
  • Neoplasms
  • Molecular Sequence Data
  • Humans