Pseudopod-like basal cell processes in intestinal cholecystokinin cells.

Journal Article (Journal Article)

Cholecystokinin (CCK) is secreted by neuroendocrine cells comprising 0.1%-0.5% of the mucosal cells in the upper small intestine. Using CCK promoter-driven green fluorescent protein (GFP) expression in transgenic mice, we have applied immunofluorescence techniques to analyze the morphology of CCK cells. GFP and CCK colocalize in neuroendocrine cells with little aberrant GFP expression. CCK-containing cells are either flask- or spindle-shaped, and in some cells, we have found dendritic processes similar to pseudopods demonstrated for gut somatostatin-containing D cells. Most pseudopods are short, the longest process visualized extending across three cells. Pseudopods usually extend to adjacent cells but some weave between neighboring cells. Dual processes have also been observed. Three-dimensional reconstructions suggest that processes are not unidirectional and thus are unlikely to be involved in migration of CCK cells from the crypt up the villus. Abundant CCK immunostaining is present in the pseudopods, suggesting that they release CCK onto the target cell. In order to identify the type of cells being targeted, we have co-stained sections with antibodies to chromogranin A, trefoil factor-3, and sucrase-isomaltase. CCK cell processes almost exclusively extend to sucrase-isomaltase-positive enterocytes. Thus, CCK cells have cellular processes possibly involved in paracrine secretion.

Full Text

Duke Authors

Cited Authors

  • Chandra, R; Samsa, LA; Vigna, SR; Liddle, RA

Published Date

  • August 2010

Published In

Volume / Issue

  • 341 / 2

Start / End Page

  • 289 - 297

PubMed ID

  • 20582553

Pubmed Central ID

  • PMC4846361

Electronic International Standard Serial Number (EISSN)

  • 1432-0878

Digital Object Identifier (DOI)

  • 10.1007/s00441-010-0997-1


  • eng

Conference Location

  • Germany