Expression of cyp26b1 during zebrafish early development.

Published

Journal Article

We have cloned the zebrafish ortholog of the mammalian cyp26b1 gene. The predicted zebrafish cyp26b1 protein shares greater than 73% identity with mammalian homologues. cDNA transfection assays showed that like human cyp26b1, zebrafish cyp26b1 is involved in limiting the activity of retinoic acid. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of embryonic RNAs suggested that no maternal cyp26b1 message is detectable. Zygotic cyp26b1 message could be detected at 75% epiboly by RT-PCR and localized to presumptive rhombomere 3 and rhombomere 4 at the early two-somite (2S) stage (10.5 hpf: hour post fertilization) by whole mount in situ hybridization. As development proceeds expression expands anteriorly to include rhombomere 2 at the 10S stage (14hpf). By 14S (16hpf) expression in the hindbrain has also expanded posteriorly and encompasses rhombomere 2 through rhombomere 6. At later stages, 24 through 48 hpf, additional expression was found in the eyes, diencephalon, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia.

Full Text

Duke Authors

Cited Authors

  • Zhao, Q; Dobbs-McAuliffe, B; Linney, E

Published Date

  • February 2005

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • 363 - 369

PubMed ID

  • 15661642

Pubmed Central ID

  • 15661642

International Standard Serial Number (ISSN)

  • 1567-133X

Digital Object Identifier (DOI)

  • 10.1016/j.modgep.2004.09.011

Language

  • eng

Conference Location

  • Netherlands