Expression of cyp26b1 during zebrafish early development.

Journal Article (Journal Article)

We have cloned the zebrafish ortholog of the mammalian cyp26b1 gene. The predicted zebrafish cyp26b1 protein shares greater than 73% identity with mammalian homologues. cDNA transfection assays showed that like human cyp26b1, zebrafish cyp26b1 is involved in limiting the activity of retinoic acid. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of embryonic RNAs suggested that no maternal cyp26b1 message is detectable. Zygotic cyp26b1 message could be detected at 75% epiboly by RT-PCR and localized to presumptive rhombomere 3 and rhombomere 4 at the early two-somite (2S) stage (10.5 hpf: hour post fertilization) by whole mount in situ hybridization. As development proceeds expression expands anteriorly to include rhombomere 2 at the 10S stage (14hpf). By 14S (16hpf) expression in the hindbrain has also expanded posteriorly and encompasses rhombomere 2 through rhombomere 6. At later stages, 24 through 48 hpf, additional expression was found in the eyes, diencephalon, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia.

Full Text

Duke Authors

Cited Authors

  • Zhao, Q; Dobbs-McAuliffe, B; Linney, E

Published Date

  • February 2005

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • 363 - 369

PubMed ID

  • 15661642

International Standard Serial Number (ISSN)

  • 1567-133X

Digital Object Identifier (DOI)

  • 10.1016/j.modgep.2004.09.011

Language

  • eng

Conference Location

  • Netherlands