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Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord.

Publication ,  Journal Article
Colbert, MC; Rubin, WW; Linney, E; LaMantia, AS
Published in: Dev Dyn
September 1995

Retinoid-dependent gene expression accompanies the emergence of distinct regions and cell classes in the mouse spinal cord around midgestation. We asked whether changes in the expression of retinoid signaling molecules and retinoid-responsive genes reflect the establishment of this regional and cellular diversity. At E10.5, retinoic acid (RA) receptors (RAR)alpha, RAR beta, the retinoid X receptor (RXR) gamma, cellular RA binding protein (CRABP)I, CRAPBII, and cellular retinol binding protein (CRBP)I mRNAs are found throughout the entire anterior-posterior (AP) axis of the cord, as is RA (Colbert et al. [1993] Proc. Natl. Acad. Sci. U.S.A. 90:6572-6576) and RA-sensitive transgene expression (Balkan et al. [1992] Proc. Natl. Acad. Sci. U.S.A. 89:3347-3351). At E12.5, RA, transgene expression, and RAR beta become restricted to the cervical and lumbar cord. RAR alpha, CRABPI, and RXR gamma, however, are found throughout the AP extent. CRABPII and CRBPI, although expanded within the cervical and lumbar regions, are also found throughout the AP axis. Thus, several retinoid signaling molecules continue to be expressed beyond distinct regions of the spinal cord where RA is available and some RA-responsive genes are either restricted or enhanced. Exogenous RA can activate a more widespread response resulting in ectopic transgene and RAR beta expression in the thoracic and sacral cord. Not all RA-sensitive genes, however, respond; CRABPII and CRBPI expression patterns are unchanged. Finally, not every cell within the normal or exogenously induced domains of RA-dependent gene expression responds to RA, nor does every cell express RA receptors or binding proteins. Thus, regional and cellular differences in the distribution of the known retinoid receptors and binding proteins do not predict absolutely where or whether retinoid sensitive genes will be expressed or where retinoids will be available in the developing spinal cord. Instead, retinoid-mediated gene expression in the cervical and lumbar cord seems to reflect retinoid responses that rely both on the local availability of retinoids, the identity of the responding gene, and an indeterminate array of retinoid signaling molecules.

Duke Scholars

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

September 1995

Volume

204

Issue

1

Start / End Page

1 / 12

Location

United States

Related Subject Headings

  • Tretinoin
  • Spinal Cord
  • Signal Transduction
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • Protein Binding
  • Mice, Transgenic
  • Mice
  • In Situ Hybridization
  • Gene Expression Regulation, Developmental
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Colbert, M. C., Rubin, W. W., Linney, E., & LaMantia, A. S. (1995). Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord. Dev Dyn, 204(1), 1–12. https://doi.org/10.1002/aja.1002040102
Colbert, M. C., W. W. Rubin, E. Linney, and A. S. LaMantia. “Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord.Dev Dyn 204, no. 1 (September 1995): 1–12. https://doi.org/10.1002/aja.1002040102.
Colbert MC, Rubin WW, Linney E, LaMantia AS. Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord. Dev Dyn. 1995 Sep;204(1):1–12.
Colbert, M. C., et al. “Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord.Dev Dyn, vol. 204, no. 1, Sept. 1995, pp. 1–12. Pubmed, doi:10.1002/aja.1002040102.
Colbert MC, Rubin WW, Linney E, LaMantia AS. Retinoid signaling and the generation of regional and cellular diversity in the embryonic mouse spinal cord. Dev Dyn. 1995 Sep;204(1):1–12.
Journal cover image

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

September 1995

Volume

204

Issue

1

Start / End Page

1 / 12

Location

United States

Related Subject Headings

  • Tretinoin
  • Spinal Cord
  • Signal Transduction
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • Protein Binding
  • Mice, Transgenic
  • Mice
  • In Situ Hybridization
  • Gene Expression Regulation, Developmental