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Transgenic indicator mice for studying activated retinoic acid receptors during development.

Publication ,  Journal Article
Balkan, W; Colbert, M; Bock, C; Linney, E
Published in: Proc Natl Acad Sci U S A
April 15, 1992

Retinoic acid (RA) receptors (RARs) are ligand-inducible transcription factors that bind to specific DNA sequences associated with the regulatory regions of RA-regulatable genes. Since RA has been implicated as an important factor both in normal development and in teratological studies, one would like to have a model system that detects the presence of activated receptors during development. We have constructed a recombinant reporter gene that has three copies of the RA response element (RARE) from the RAR beta-2 promoter 5' to the herpes simplex virus thymidine kinase promoter; this regulatory region is coupled to the bacterial beta-galactosidase reporter gene. This construct was RA inducible in transient transfection assays in F9 embryonal carcinoma cells. Transgenic embryos with this reporter gene construct exhibited restricted and reproducible patterns of beta-galactosidase activity during embryogenesis, beginning between gestational ages day 7.5 and 8.5. At day 8.5, beta-galactosidase activity was detected in the closed neurotube and somites. Day 8.5 embryos, from pregnant females fed RA 14 hr earlier, exhibited a greater intensity and distribution of beta-galactosidase activity. Similarly, at later stages of gestation, maternal RA exposure resulted in enhanced embryonic beta-galactosidase expression. This type of transgenic indicator mouse should be useful in detailing the role of activated RARs during embryonic development.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 15, 1992

Volume

89

Issue

8

Start / End Page

3347 / 3351

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Tretinoin
  • Transfection
  • Thymidine Kinase
  • Simplexvirus
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Promoter Regions, Genetic
  • Pregnancy
 

Citation

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Balkan, W., Colbert, M., Bock, C., & Linney, E. (1992). Transgenic indicator mice for studying activated retinoic acid receptors during development. Proc Natl Acad Sci U S A, 89(8), 3347–3351. https://doi.org/10.1073/pnas.89.8.3347
Balkan, W., M. Colbert, C. Bock, and E. Linney. “Transgenic indicator mice for studying activated retinoic acid receptors during development.Proc Natl Acad Sci U S A 89, no. 8 (April 15, 1992): 3347–51. https://doi.org/10.1073/pnas.89.8.3347.
Balkan W, Colbert M, Bock C, Linney E. Transgenic indicator mice for studying activated retinoic acid receptors during development. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3347–51.
Balkan, W., et al. “Transgenic indicator mice for studying activated retinoic acid receptors during development.Proc Natl Acad Sci U S A, vol. 89, no. 8, Apr. 1992, pp. 3347–51. Pubmed, doi:10.1073/pnas.89.8.3347.
Balkan W, Colbert M, Bock C, Linney E. Transgenic indicator mice for studying activated retinoic acid receptors during development. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3347–3351.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 15, 1992

Volume

89

Issue

8

Start / End Page

3347 / 3351

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Tretinoin
  • Transfection
  • Thymidine Kinase
  • Simplexvirus
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Promoter Regions, Genetic
  • Pregnancy