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Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells.

Publication ,  Journal Article
Espeseth, AS; Murphy, SP; Linney, E
Published in: Genes Dev
November 1989

Expression vectors have been constructed for a region of the human retinoic acid receptor-alpha (hRAR-alpha) and transferred into F9 embryonal carcinoma (EC) cells. When the vectors are overexpressed in F9 cells, clones can be selected for resistance to retinoic acid-induced differentiation. This effect is obtained even when the hRAR-alpha region is expressed as a beta-galactosidase fusion protein. Using the beta-galactosidase component of the fusion protein as a marker, overexpression of the fusion protein has been correlated with the retinoic acid-resistance effect. The clones resistant to retinoic acid no longer exhibit the normal retinoic acid induction of endo B cytokeratin, laminin B-1, and tissue plasminogen activator mRNAs observed with normal F9 cells. Retinoic acid induction of type IV alpha-1 collagen and Hox-1.3 RNAs is observed with these clones. When transfected with a thyroid receptor DNA-binding sequence (TRE)/thymidine kinase promoter/luciferase construct, the retinoic acid-resistant clones do not yield the same retinoic acid-induced level of luciferase obtained with F9 cells. It is hypothesized that the RAR vectors are interfering with endogenous RAR(s) in a dominant-negative manner to inhibit retinoic acid-induced differentiation of F9 EC cells.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

November 1989

Volume

3

Issue

11

Start / End Page

1647 / 1656

Location

United States

Related Subject Headings

  • Tretinoin
  • Transfection
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Phenotype
  • Neoplastic Stem Cells
  • Humans
  • Genetic Vectors
  • Gene Expression
  • Embryonal Carcinoma Stem Cells
 

Citation

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Espeseth, A. S., Murphy, S. P., & Linney, E. (1989). Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells. Genes Dev, 3(11), 1647–1656. https://doi.org/10.1101/gad.3.11.1647
Espeseth, A. S., S. P. Murphy, and E. Linney. “Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells.Genes Dev 3, no. 11 (November 1989): 1647–56. https://doi.org/10.1101/gad.3.11.1647.
Espeseth AS, Murphy SP, Linney E. Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells. Genes Dev. 1989 Nov;3(11):1647–56.
Espeseth, A. S., et al. “Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells.Genes Dev, vol. 3, no. 11, Nov. 1989, pp. 1647–56. Pubmed, doi:10.1101/gad.3.11.1647.
Espeseth AS, Murphy SP, Linney E. Retinoic acid receptor expression vector inhibits differentiation of F9 embryonal carcinoma cells. Genes Dev. 1989 Nov;3(11):1647–1656.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

November 1989

Volume

3

Issue

11

Start / End Page

1647 / 1656

Location

United States

Related Subject Headings

  • Tretinoin
  • Transfection
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Phenotype
  • Neoplastic Stem Cells
  • Humans
  • Genetic Vectors
  • Gene Expression
  • Embryonal Carcinoma Stem Cells