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Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells.

Publication ,  Journal Article
Murphy, SP; Garbern, J; Odenwald, WF; Lazzarini, RA; Linney, E
Published in: Proc Natl Acad Sci U S A
August 1988

The Hox-1.3 gene is located on mouse chromosome 6 and has been previously shown to be expressed in mouse embryos and adults. In this study, we have examined the steady-state levels of the Hox-1.3 transcripts in undifferentiated and differentiated F9 embryonal carcinoma cells. We find that there is a rapid increase of Hox-1.3 transcripts after differentiation induction of F9 cells. The level of the major 1.85-kilobase (kb) transcript peaks at 16-24 hr after differentiation induction of F9 cells. By using primer extension techniques the 5' ends of the major 1.85-kb transcript have been mapped to two sites in induced F9 cells. Cellular fractionation of RNA and transfer blot gel analysis has localized one minor transcript to the nucleus, whereas the major transcript and two additional minor transcripts appear in the nucleus and the cytoplasm of induced F9 cells. The results of nuclear run-off experiments with uninduced and induced F9 cell nuclei indicate that there is a substantial increase in the rate of Hox-1.3 transcription upon induction of F9 cells with retinoic acid.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 1988

Volume

85

Issue

15

Start / End Page

5587 / 5591

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Teratoma
  • RNA
  • Nucleic Acid Hybridization
  • Neoplastic Stem Cells
  • Genes, Homeobox
  • Gene Expression Regulation
  • Embryonal Carcinoma Stem Cells
  • Densitometry
 

Citation

APA
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MLA
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Murphy, S. P., Garbern, J., Odenwald, W. F., Lazzarini, R. A., & Linney, E. (1988). Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells. Proc Natl Acad Sci U S A, 85(15), 5587–5591. https://doi.org/10.1073/pnas.85.15.5587
Murphy, S. P., J. Garbern, W. F. Odenwald, R. A. Lazzarini, and E. Linney. “Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells.Proc Natl Acad Sci U S A 85, no. 15 (August 1988): 5587–91. https://doi.org/10.1073/pnas.85.15.5587.
Murphy SP, Garbern J, Odenwald WF, Lazzarini RA, Linney E. Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5587–91.
Murphy, S. P., et al. “Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells.Proc Natl Acad Sci U S A, vol. 85, no. 15, Aug. 1988, pp. 5587–91. Pubmed, doi:10.1073/pnas.85.15.5587.
Murphy SP, Garbern J, Odenwald WF, Lazzarini RA, Linney E. Differential expression of the homeobox gene Hox-1.3 in F9 embryonal carcinoma cells. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5587–5591.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

August 1988

Volume

85

Issue

15

Start / End Page

5587 / 5591

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Teratoma
  • RNA
  • Nucleic Acid Hybridization
  • Neoplastic Stem Cells
  • Genes, Homeobox
  • Gene Expression Regulation
  • Embryonal Carcinoma Stem Cells
  • Densitometry