Suppression of endo B cytokeratin by its antisense RNA inhibits the normal coexpression of endo A cytokeratin.


Journal Article

Antisense endo B cytokeratin RNA encoded by a retrovirus vector was expressed in a derivative of the F9 embryonal carcinoma cell line. Two G418-resistant clones were selected that expressed a colinear transcript containing both neomycin and antisense endo B cytokeratin sequences. Expression of a 5-fold excess of antisense endo B RNA over endogenous, retinoic acid-induced endo B RNA resulted in suppression of endo B cytokeratin protein expression. In addition, the normal induction of endo A protein, the type II cytokeratin that polymerizes with endo B, was suppressed at the RNA and protein levels. Revertant clones, which synthesize little if any neo or antisense endo B RNA, regain the ability to express the affected gene products in response to retinoic acid. These results indicate that the suppression of endo B cytokeratin protein synthesis influences the stable levels of endo A mRNA.

Full Text

Duke Authors

Cited Authors

  • Trevor, K; Linney, E; Oshima, RG

Published Date

  • February 1, 1987

Published In

Volume / Issue

  • 84 / 4

Start / End Page

  • 1040 - 1044

PubMed ID

  • 2434948

Pubmed Central ID

  • 2434948

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.4.1040


  • eng

Conference Location

  • United States