Skip to main content

Retroviral vector gene expression in F9 embryonal carcinoma cells.

Publication ,  Journal Article
Linney, E; Neill, SD; Prestridge, DS
Published in: J Virol
October 1987

When F9 embryonal carcinoma (EC) cells are infected with retroviral vectors, the efficiency of expression of selectable genes is considerably lower than that in mouse fibroblasts infected with the same retroviral vectors. In this study, several retroviral vectors with regulatory sequences placed immediately 5' to a selectable gene were constructed, packaged, and used to infect mouse fibroblasts and F9 EC cells. With selection as an assay, there was a hierarchy of relative expression in F9 cells compared with that in mouse fibroblasts. These internally placed regulatory sequences are the source of the mRNAs detected in F9 EC cells, while both retroviral long-terminal-repeat promoters and internal promoters are the source of steady-state mRNAs in mouse fibroblasts. This effect was observable with both the internally placed herpes simplex virus thymidine kinase promoter and the Moloney murine leukemia virus promoter.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1987

Volume

61

Issue

10

Start / End Page

3248 / 3253

Location

United States

Related Subject Headings

  • Virology
  • Transcription, Genetic
  • Thymidine Kinase
  • Simplexvirus
  • Retroviridae
  • Repetitive Sequences, Nucleic Acid
  • RNA, Viral
  • Promoter Regions, Genetic
  • Nucleic Acid Hybridization
  • Neoplastic Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Linney, E., Neill, S. D., & Prestridge, D. S. (1987). Retroviral vector gene expression in F9 embryonal carcinoma cells. J Virol, 61(10), 3248–3253. https://doi.org/10.1128/JVI.61.10.3248-3253.1987
Linney, E., S. D. Neill, and D. S. Prestridge. “Retroviral vector gene expression in F9 embryonal carcinoma cells.J Virol 61, no. 10 (October 1987): 3248–53. https://doi.org/10.1128/JVI.61.10.3248-3253.1987.
Linney E, Neill SD, Prestridge DS. Retroviral vector gene expression in F9 embryonal carcinoma cells. J Virol. 1987 Oct;61(10):3248–53.
Linney, E., et al. “Retroviral vector gene expression in F9 embryonal carcinoma cells.J Virol, vol. 61, no. 10, Oct. 1987, pp. 3248–53. Pubmed, doi:10.1128/JVI.61.10.3248-3253.1987.
Linney E, Neill SD, Prestridge DS. Retroviral vector gene expression in F9 embryonal carcinoma cells. J Virol. 1987 Oct;61(10):3248–3253.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1987

Volume

61

Issue

10

Start / End Page

3248 / 3253

Location

United States

Related Subject Headings

  • Virology
  • Transcription, Genetic
  • Thymidine Kinase
  • Simplexvirus
  • Retroviridae
  • Repetitive Sequences, Nucleic Acid
  • RNA, Viral
  • Promoter Regions, Genetic
  • Nucleic Acid Hybridization
  • Neoplastic Stem Cells