Retroviral vector gene expression in F9 embryonal carcinoma cells.

Published

Journal Article

When F9 embryonal carcinoma (EC) cells are infected with retroviral vectors, the efficiency of expression of selectable genes is considerably lower than that in mouse fibroblasts infected with the same retroviral vectors. In this study, several retroviral vectors with regulatory sequences placed immediately 5' to a selectable gene were constructed, packaged, and used to infect mouse fibroblasts and F9 EC cells. With selection as an assay, there was a hierarchy of relative expression in F9 cells compared with that in mouse fibroblasts. These internally placed regulatory sequences are the source of the mRNAs detected in F9 EC cells, while both retroviral long-terminal-repeat promoters and internal promoters are the source of steady-state mRNAs in mouse fibroblasts. This effect was observable with both the internally placed herpes simplex virus thymidine kinase promoter and the Moloney murine leukemia virus promoter.

Full Text

Duke Authors

Cited Authors

  • Linney, E; Neill, SD; Prestridge, DS

Published Date

  • October 1, 1987

Published In

Volume / Issue

  • 61 / 10

Start / End Page

  • 3248 - 3253

PubMed ID

  • 3041045

Pubmed Central ID

  • 3041045

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States