High throughput screening for neurodegeneration and complex disease phenotypes.


Journal Article (Review)

High throughput screening (HTS) for complex diseases is challenging. This stems from the fact that complex phenotypes are difficult to adapt to rapid, high throughput assays. We describe the recent development of high throughput and high-content screens (HCS) for neurodegenerative diseases, with a focus on inherited neurodegenerative disorders, such as Huntington's disease. We describe, among others, HTS assays based on protein aggregation, neuronal death, caspase activation and mutant protein clearance. Furthermore, we describe high-content screens that are being used to prioritize hits identified in such HTS assays. These assays and screening approaches should accelerate drug discovery for neurodegenerative disorders and guide the development of screening approaches for other complex disease phenotypes.

Full Text

Cited Authors

  • Varma, H; Lo, DC; Stockwell, BR

Published Date

  • March 2008

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 238 - 248

PubMed ID

  • 18336216

Pubmed Central ID

  • 18336216

Electronic International Standard Serial Number (EISSN)

  • 1875-5402

International Standard Serial Number (ISSN)

  • 1386-2073

Digital Object Identifier (DOI)

  • 10.2174/138620708783877753


  • eng