Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes.

Published

Journal Article

The bioflavinoid quercetin was found to exert at least three separate effects on human polymorphonuclear leukocytes. (1) Concentrations of approximately 100 microM inhibited the membrane-associated magnesium adenosine triphosphatase by 60%-80% in either broken cell preparations or intact cells. Lineweaver-Burk plots showed the inhibition to be uncompetitive in nature. (2) Similar concentrations of quercetin inhibited respiratory burst activity of the cells as measured by oxygen consumption, glucose oxidation, or iodination of protein. All inhibitions were dose-dependent and were observed with either opsonized zymosan or phorbol myristate acetate as stimulus. (3) Quercetin likewise inhibited the transport of the nonmetabolizable hexose, 3H-2-deoxyglucose. These observations are most consistent with the hypothesis that quercetin exerts a generalized effect at the level of the cell membrane of the neutrophil.

Full Text

Duke Authors

Cited Authors

  • Long, GD; DeChatelet, LR; O'Flaherty, JT; McCall, CE; Bass, DA; Shirley, PS; Parce, JW

Published Date

  • March 1, 1981

Published In

Volume / Issue

  • 57 / 3

Start / End Page

  • 561 - 566

PubMed ID

  • 6450623

Pubmed Central ID

  • 6450623

International Standard Serial Number (ISSN)

  • 0006-4971

Language

  • eng

Conference Location

  • United States