Activation of transgene expression in skeletal muscle by focused ultrasound.
Published
Journal Article
To correlate thermal dose from focused ultrasound (FUS) with gene expression and tissue injury, a temperature plateau strategy was employed. Plasmids encoding luciferase gene under the control of hsp70B promoter were transfected into the right gastrocnemius muscle in a rat via electroporation. One day after transfection, hind limbs were treated with 3.3-MHz focused ultrasound, using one of four different temperature plateaus with spatial-peak time-average focal temperatures (T(SPTA)) of 46 degrees C, 48 degrees C, 51 degrees C and 62 degrees C. The treatment duration at the plateau temperature was varied from 0 to 30s. Gene expression was analyzed in vivo one day following FUS treatment, and H&E staining was employed to assess tissue injury. Gene activation and tissue damage correlated closely with thermal dose. The highest level of gene activation was induced by FUS at T(SPTA)=51 degrees C for 20s, which was found to be statistically equivalent to that produced by water-bath hyperthermia.
Full Text
Duke Authors
Cited Authors
- Lu, X; Sankin, G; Pua, EC; Madden, J; Zhong, P
Published Date
- February 6, 2009
Published In
Volume / Issue
- 379 / 2
Start / End Page
- 428 - 433
PubMed ID
- 19118526
Pubmed Central ID
- 19118526
Electronic International Standard Serial Number (EISSN)
- 1090-2104
Digital Object Identifier (DOI)
- 10.1016/j.bbrc.2008.12.090
Language
- eng
Conference Location
- United States