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Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore.

Publication ,  Journal Article
Macklis, JD; Madison, RD
Published in: J Neurosci
July 1991

Studies of neural cell transplantation would be aided by the ability to damage or destroy, noninvasively and extremely selectively, grafted cells at defined times following their initial implantation. Mechanisms of graft integration and performance could be investigated, also providing insight into natural injury and repair mechanisms. At long wavelengths between 650 and 850 nm, laser energy can penetrate several millimeters of brain tissue without absorption or damage to the unpigmented tissue. However, targeted cells are selectively damaged by illumination at these long wavelengths if they contain latex nanospheres with incorporated cytolytic chromophores (e.g., chlorin e6). Light penetration allows many thousands of cells to be lesioned simultaneously, noninvasively, and deep within a surrounding matrix of other tissue. Such laser-activated damage has been termed laser photolysis (PL). We studied damage to C1300 neuroblastoma (NB) cells grafted into mouse neocortex in vivo by this process of PL. NB cells provided a simple and reproducible model of neural grafting, allowing direct histologic assessment of cellular growth and viability by distinct morphologic and mitotic criteria. Cells were cultured by standard methods, labeled in vitro by brief exposure to nanospheres containing chlorin e6, and grafted to sites within deep layers of mouse neocortex. Mice were exposed to transcranial, fractionated, unfocused pulses of 670-nm-wavelength energy totaling 90-120 J/cm2. We histologically assessed graft growth and cellular viability over a period from 2 d to 4 weeks, measured graft volumes quantitatively during the period of early rapid growth in controls (2 and 7 d), and generated 3-D reconstructions from serial sections to assist in visual analysis.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

July 1991

Volume

11

Issue

7

Start / End Page

2055 / 2062

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Porphyrins
  • Photolysis
  • Neurons
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Neoplasm Transplantation
  • Microspheres
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
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ICMJE
MLA
NLM
Macklis, J. D., & Madison, R. D. (1991). Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 11(7), 2055–2062. https://doi.org/10.1523/jneurosci.11-07-02055.1991
Macklis, J. D., and R. D. Madison. “Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore.The Journal of Neuroscience : The Official Journal of the Society for Neuroscience 11, no. 7 (July 1991): 2055–62. https://doi.org/10.1523/jneurosci.11-07-02055.1991.
Macklis JD, Madison RD. Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1991 Jul;11(7):2055–62.
Macklis, J. D., and R. D. Madison. “Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore.The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, vol. 11, no. 7, July 1991, pp. 2055–62. Epmc, doi:10.1523/jneurosci.11-07-02055.1991.
Macklis JD, Madison RD. Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1991 Jul;11(7):2055–2062.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

July 1991

Volume

11

Issue

7

Start / End Page

2055 / 2062

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Porphyrins
  • Photolysis
  • Neurons
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Neoplasm Transplantation
  • Microspheres
  • Mice, Inbred C57BL
  • Mice