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Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway.

Publication ,  Journal Article
Seubert, J; Yang, B; Bradbury, JA; Graves, J; Degraff, LM; Gabel, S; Gooch, R; Foley, J; Newman, J; Mao, L; Rockman, HA; Hammock, BD ...
Published in: Circ Res
September 3, 2004

Human CYP2J2 is abundant in heart and active in the biosynthesis of epoxyeicosatrienoic acids (EETs); however, the functional role of this P450 and its eicosanoid products in the heart remains unknown. Transgenic mice with cardiomyocyte-specific overexpression of CYP2J2 were generated. CYP2J2 transgenic (Tr) mice have normal heart anatomy and basal contractile function. CYP2J2 Tr hearts have improved recovery of left ventricular developed pressure (LVDP) compared with wild-type (WT) hearts after 20 minutes ischemia and 40 minutes reperfusion. Perfusion with the selective P450 epoxygenase inhibitor N-methylsulphonyl-6-(2-proparglyloxyphenyl)hexanamide (MS-PPOH) for 20 minutes before ischemia results in reduced postischemic LVDP recovery in WT hearts and abolishes the improved postischemic LVDP recovery in CYP2J2 Tr hearts. Perfusion with the ATP-sensitive K(+) channel (K(ATP)) inhibitor glibenclamide (GLIB) or the mitochondrial K(ATP) (mitoK(ATP)) inhibitor 5-hydroxydecanoate (5-HD) for 20 minutes before ischemia abolishes the cardioprotective effects of CYP2J2 overexpression. Flavoprotein fluorescence, a marker of mitoK(ATP) activity, is higher in cardiomyocytes from CYP2J2 Tr versus WT mice. Moreover, CYP2J2-derived EETs (1 to 5 micromol/L) increase flavoprotein fluorescence in WT cardiomyocytes. CYP2J2 Tr mice exhibit increased expression of phospho-p42/p44 mitogen-activated protein kinase (MAPK) after ischemia, and addition of the p42/p44 MAPK kinase (MEK) inhibitor PD98059 during reperfusion abolishes the cardioprotective effects of CYP2J2 overexpression. Together, these data suggest that CYP2J2-derived metabolites are cardioprotective after ischemia, and the mechanism for this cardioprotection involves activation of mitoK(ATP) and p42/p44 MAPK.

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Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

September 3, 2004

Volume

95

Issue

5

Start / End Page

506 / 514

Location

United States

Related Subject Headings

  • Ventricular Pressure
  • Ventricular Function, Left
  • Potassium Channels
  • Potassium Channel Blockers
  • Oxygenases
  • Myocardium
  • Myocardial Reperfusion Injury
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinase 1
  • Mice, Transgenic
 

Citation

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MLA
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Seubert, J., Yang, B., Bradbury, J. A., Graves, J., Degraff, L. M., Gabel, S., … Zeldin, D. C. (2004). Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway. Circ Res, 95(5), 506–514. https://doi.org/10.1161/01.RES.0000139436.89654.c8
Seubert, John, Baichun Yang, J Alyce Bradbury, Joan Graves, Laura M. Degraff, Scott Gabel, Rebecca Gooch, et al. “Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway.Circ Res 95, no. 5 (September 3, 2004): 506–14. https://doi.org/10.1161/01.RES.0000139436.89654.c8.
Seubert J, Yang B, Bradbury JA, Graves J, Degraff LM, Gabel S, et al. Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway. Circ Res. 2004 Sep 3;95(5):506–14.
Seubert, John, et al. “Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway.Circ Res, vol. 95, no. 5, Sept. 2004, pp. 506–14. Pubmed, doi:10.1161/01.RES.0000139436.89654.c8.
Seubert J, Yang B, Bradbury JA, Graves J, Degraff LM, Gabel S, Gooch R, Foley J, Newman J, Mao L, Rockman HA, Hammock BD, Murphy E, Zeldin DC. Enhanced postischemic functional recovery in CYP2J2 transgenic hearts involves mitochondrial ATP-sensitive K+ channels and p42/p44 MAPK pathway. Circ Res. 2004 Sep 3;95(5):506–514.

Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

September 3, 2004

Volume

95

Issue

5

Start / End Page

506 / 514

Location

United States

Related Subject Headings

  • Ventricular Pressure
  • Ventricular Function, Left
  • Potassium Channels
  • Potassium Channel Blockers
  • Oxygenases
  • Myocardium
  • Myocardial Reperfusion Injury
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinase 1
  • Mice, Transgenic