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No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation.

Publication ,  Journal Article
Pittman, KM; Losken, HW; Kleinman, ME; Marcus, JR; Blei, F; Gurtner, GC; Marchuk, DA
Published in: J Invest Dermatol
November 2006

In this study, using the placental origin theory as a basis, we set out to explore whether hemangioma endothelial cells (HEC) were maternal in origin. We rigorously addressed this hypothesis using several molecular genetic techniques. Fluorescent in situ hybridization on surgical specimens of proliferating hemangiomas (n=8) demonstrated no XX-labeled HEC from resected tumors of male infants. This analysis was followed by PCR genotyping of HEC (n=11) using microsatellite markers where cellular components were genotyped and compared to genomic DNA of corresponding mother-child pairs. In the seven informative mother-child pairs, HEC matched the genotype of the child and not the maternal genotype. Concerned that HEC represented a mixed population of cells, we subsequently enriched for cells using the placental-specific endothelial cell (EC) marker, Fc gammaRII. Three informative mother-child pairs exhibited only the genotype of the child in our enriched cell population. Using sequence analysis, we identified an informative single nucleotide polymorphism in an exon of the placental-EC-specific protein, GLUT1. When comparing GLUT1 complementary DNA (cDNA) with mother-child DNA, the genotype of the cDNA matched the constitutional DNA of the child. Our results indicate that hemangiomas are not microchimeric in origin. This study provides further insight into the origin of a tumor whose pathogenesis remains elusive.

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Published In

J Invest Dermatol

DOI

EISSN

1523-1747

Publication Date

November 2006

Volume

126

Issue

11

Start / End Page

2533 / 2538

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Receptors, IgG
  • Pregnancy
  • Polymorphism, Single Nucleotide
  • Polymerase Chain Reaction
  • Placenta
  • Microsatellite Repeats
  • Male
  • Infant
  • In Situ Hybridization, Fluorescence
 

Citation

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ICMJE
MLA
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Pittman, K. M., Losken, H. W., Kleinman, M. E., Marcus, J. R., Blei, F., Gurtner, G. C., & Marchuk, D. A. (2006). No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation. J Invest Dermatol, 126(11), 2533–2538. https://doi.org/10.1038/sj.jid.5700516
Pittman, Kristianna M., H Wolfgang Losken, Mark E. Kleinman, Jeffrey R. Marcus, Francine Blei, Geoffrey C. Gurtner, and Douglas A. Marchuk. “No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation.J Invest Dermatol 126, no. 11 (November 2006): 2533–38. https://doi.org/10.1038/sj.jid.5700516.
Pittman KM, Losken HW, Kleinman ME, Marcus JR, Blei F, Gurtner GC, et al. No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation. J Invest Dermatol. 2006 Nov;126(11):2533–8.
Pittman, Kristianna M., et al. “No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation.J Invest Dermatol, vol. 126, no. 11, Nov. 2006, pp. 2533–38. Pubmed, doi:10.1038/sj.jid.5700516.
Pittman KM, Losken HW, Kleinman ME, Marcus JR, Blei F, Gurtner GC, Marchuk DA. No evidence for maternal-fetal microchimerism in infantile hemangioma: a molecular genetic investigation. J Invest Dermatol. 2006 Nov;126(11):2533–2538.
Journal cover image

Published In

J Invest Dermatol

DOI

EISSN

1523-1747

Publication Date

November 2006

Volume

126

Issue

11

Start / End Page

2533 / 2538

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Receptors, IgG
  • Pregnancy
  • Polymorphism, Single Nucleotide
  • Polymerase Chain Reaction
  • Placenta
  • Microsatellite Repeats
  • Male
  • Infant
  • In Situ Hybridization, Fluorescence