High speed nonlinear interferometric vibrational analysis of lipids by spectral decomposition.


Journal Article

Unlike other CARS-based (coherent anti-Stokes Raman scattering) spectroscopy techniques, nonlinear interferometric vibrational spectroscopy (NIVS) is linear in analyte concentration and has a Raman line shape free of nonresonant background distortions. We use spontaneous Raman scattering as a high accuracy benchmark for NIVS. As a challenging comparison, we examine spectra in the CH stretching region of six lipid samples. Singular value decomposition and reference to an independent chemical assay are used to directly compare NIVS and spontaneous Raman scattering. We demonstrate that NIVS can determine the relative degree of unsaturation in six different lipid samples as accurately as spontaneous Raman spectroscopy, but 200 times faster. A skin tissue sample is mapped out to demonstrate quantitative lipid-protein differentiation with spatial resolution.

Full Text

Duke Authors

Cited Authors

  • Chowdary, PD; Benalcazar, WA; Jiang, Z; Marks, DM; Boppart, SA; Gruebele, M

Published Date

  • May 2010

Published In

Volume / Issue

  • 82 / 9

Start / End Page

  • 3812 - 3818

PubMed ID

  • 20373786

Pubmed Central ID

  • 20373786

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac100222c


  • eng