Protein microarray signature of autoantibody biomarkers for the early detection of breast cancer.

Journal Article (Journal Article)

Cancer patients spontaneously generate autoantibodies (AAb) to tumor-derived proteins. To detect AAb, we have probed novel high-density custom protein microarrays (NAPPA) expressing 4988 candidate tumor antigens with sera from patients with early stage breast cancer (IBC), and bound IgG was measured. We used a three-phase serial screening approach. First, a prescreen was performed to eliminate uninformative antigens. Sera from stage I-III IBC (n = 53) and healthy women (n = 53) were screened for AAb to all 4988 protein antigens. Antigens were selected if the 95th percentile of signal of cases and controls were significantly different (p < 0.05) and if the number of cases with signals above the 95th percentile of controls was significant (p < 0.05). These 761 antigens were screened using an independent set of IBC sera (n = 51) and sera from women with benign breast disease (BBD) (n = 39). From these, 119 antigens had a partial area under the ROC curve (p < 0.05), with sensitivities ranging from 9-40% at >91% specificity. Twenty-eight of these antigens were confirmed using an independent serum cohort (n = 51 cases/38 controls, p < 0.05). Using all 28 AAb, a classifier was identified with a sensitivity of 80.8% and a specificity of 61.6% (AUC = 0.756). These are potential biomarkers for the early detection of breast cancer.

Full Text

Duke Authors

Cited Authors

  • Anderson, KS; Sibani, S; Wallstrom, G; Qiu, J; Mendoza, EA; Raphael, J; Hainsworth, E; Montor, WR; Wong, J; Park, JG; Lokko, N; Logvinenko, T; Ramachandran, N; Godwin, AK; Marks, J; Engstrom, P; Labaer, J

Published Date

  • January 7, 2011

Published In

Volume / Issue

  • 10 / 1

Start / End Page

  • 85 - 96

PubMed ID

  • 20977275

Pubmed Central ID

  • PMC3158028

Electronic International Standard Serial Number (EISSN)

  • 1535-3907

Digital Object Identifier (DOI)

  • 10.1021/pr100686b


  • eng

Conference Location

  • United States