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Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation.

Publication ,  Journal Article
Evron, E; Umbricht, CB; Korz, D; Raman, V; Loeb, DM; Niranjan, B; Buluwela, L; Weitzman, SA; Marks, J; Sukumar, S
Published in: Cancer Res
March 15, 2001

Cyclin D2 is a member of the D-type cyclins, implicated in cell cycle regulation, differentiation, and malignant transformation. It was noted previously that cyclin D2 is not expressed in the majority of breast cancer cell lines, whereas abundant expression was detected in finite life span human mammary epithelial cells. By reverse transcription-PCR and Western blot analysis, we extended this finding to primary breast carcinomas and show that the majority of these tumors lack expression of cyclin D2 mRNA (18 of 24) and protein (10 of 13). In contrast, both luminal and myoepithelial subpopulations of normal breast tissues expressed cyclin D2. Hypermethylation of the CpG island in the promoter was detected by methylation-specific PCR in nearly half of the breast cancers (49 of 106) and was associated with silencing of cyclin D2 gene expression. Promoter hypermethylation was also detected in ductal carcinoma in situ, suggesting that loss of cyclin D2 expression is an early event in tumorigenesis. Our results suggest that loss of cyclin D2 expression is associated with the evolution of breast cancer.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 15, 2001

Volume

61

Issue

6

Start / End Page

2782 / 2787

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Humans
  • Gene Silencing
  • Gene Expression Regulation, Neoplastic
  • Female
  • DNA Methylation
  • Cyclins
 

Citation

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MLA
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Evron, E., Umbricht, C. B., Korz, D., Raman, V., Loeb, D. M., Niranjan, B., … Sukumar, S. (2001). Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. Cancer Res, 61(6), 2782–2787.
Evron, E., C. B. Umbricht, D. Korz, V. Raman, D. M. Loeb, B. Niranjan, L. Buluwela, S. A. Weitzman, J. Marks, and S. Sukumar. “Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation.Cancer Res 61, no. 6 (March 15, 2001): 2782–87.
Evron E, Umbricht CB, Korz D, Raman V, Loeb DM, Niranjan B, et al. Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. Cancer Res. 2001 Mar 15;61(6):2782–7.
Evron, E., et al. “Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation.Cancer Res, vol. 61, no. 6, Mar. 2001, pp. 2782–87.
Evron E, Umbricht CB, Korz D, Raman V, Loeb DM, Niranjan B, Buluwela L, Weitzman SA, Marks J, Sukumar S. Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. Cancer Res. 2001 Mar 15;61(6):2782–2787.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

March 15, 2001

Volume

61

Issue

6

Start / End Page

2782 / 2787

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Humans
  • Gene Silencing
  • Gene Expression Regulation, Neoplastic
  • Female
  • DNA Methylation
  • Cyclins