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Loss of chromosome 8p sequences in human breast carcinoma cell lines.

Publication ,  Journal Article
Pykett, MJ; Murphy, ME; Harnish, PR; Muenke, M; Marks, J; George, DL
Published in: Cancer Genet Cytogenet
August 1994

Cytogenetic and molecular analyses of human breast cancer cells have identified consistent losses of specific chromosomal regions in these tumors, suggesting that such regions harbor tumor suppressor genes whose homozygous loss or inactivation directly contributes to tumorigenesis. To date, deletions of chromosome 8 sequences have been described infrequently and only in low percentages of breast carcinomas. We report the identification of a new DNA marker on chromosome 8p that is deleted in 6 (75%) of 8 breast carcinoma cell lines and in 1 primary breast carcinoma examined. No deletion of this marker was detected in any normal or nonbreast carcinoma cell lines analyzed. Southern blot and fluorescence in situ hybridization studies indicate that this clone maps to chromosome 8 between bands p12 and p21. These observations suggest that a new gene, whose loss or inactivation may foster breast carcinoma tumorigenesis, may reside in this chromosome 8p region.

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Published In

Cancer Genet Cytogenet

DOI

ISSN

0165-4608

Publication Date

August 1994

Volume

76

Issue

1

Start / End Page

23 / 28

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Deletion
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • In Situ Hybridization, Fluorescence
  • Humans
  • DNA Probes
  • Chromosomes, Human, Pair 8
  • Chromosome Mapping
  • Breast Neoplasms
 

Citation

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Pykett, M. J., Murphy, M. E., Harnish, P. R., Muenke, M., Marks, J., & George, D. L. (1994). Loss of chromosome 8p sequences in human breast carcinoma cell lines. Cancer Genet Cytogenet, 76(1), 23–28. https://doi.org/10.1016/0165-4608(94)90064-7
Pykett, M. J., M. E. Murphy, P. R. Harnish, M. Muenke, J. Marks, and D. L. George. “Loss of chromosome 8p sequences in human breast carcinoma cell lines.Cancer Genet Cytogenet 76, no. 1 (August 1994): 23–28. https://doi.org/10.1016/0165-4608(94)90064-7.
Pykett MJ, Murphy ME, Harnish PR, Muenke M, Marks J, George DL. Loss of chromosome 8p sequences in human breast carcinoma cell lines. Cancer Genet Cytogenet. 1994 Aug;76(1):23–8.
Pykett, M. J., et al. “Loss of chromosome 8p sequences in human breast carcinoma cell lines.Cancer Genet Cytogenet, vol. 76, no. 1, Aug. 1994, pp. 23–28. Pubmed, doi:10.1016/0165-4608(94)90064-7.
Pykett MJ, Murphy ME, Harnish PR, Muenke M, Marks J, George DL. Loss of chromosome 8p sequences in human breast carcinoma cell lines. Cancer Genet Cytogenet. 1994 Aug;76(1):23–28.
Journal cover image

Published In

Cancer Genet Cytogenet

DOI

ISSN

0165-4608

Publication Date

August 1994

Volume

76

Issue

1

Start / End Page

23 / 28

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Deletion
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • In Situ Hybridization, Fluorescence
  • Humans
  • DNA Probes
  • Chromosomes, Human, Pair 8
  • Chromosome Mapping
  • Breast Neoplasms