A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.

Published

Journal Article

Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.

Full Text

Duke Authors

Cited Authors

  • DasGupta, R; Nybakken, K; Booker, M; Mathey-Prevot, B; Gonsalves, F; Changkakoty, B; Perrimon, N

Published Date

  • 2007

Published In

Volume / Issue

  • 8 / 9

Start / End Page

  • R203 -

PubMed ID

  • 17903264

Pubmed Central ID

  • 17903264

Electronic International Standard Serial Number (EISSN)

  • 1474-760X

Digital Object Identifier (DOI)

  • 10.1186/gb-2007-8-9-r203

Language

  • eng

Conference Location

  • England