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A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.

Publication ,  Journal Article
DasGupta, R; Nybakken, K; Booker, M; Mathey-Prevot, B; Gonsalves, F; Changkakoty, B; Perrimon, N
Published in: Genome biology
January 2007

Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.

Duke Scholars

Published In

Genome biology

DOI

EISSN

1474-760X

ISSN

1474-7596

Publication Date

January 2007

Volume

8

Issue

9

Start / End Page

R203

Related Subject Headings

  • Transcription, Genetic
  • Sensitivity and Specificity
  • RNA, Double-Stranded
  • RNA Interference
  • RNA
  • Models, Genetic
  • Genome
  • Genetic Techniques
  • Genes, Insect
  • Gene Library
 

Citation

APA
Chicago
ICMJE
MLA
NLM
DasGupta, R., Nybakken, K., Booker, M., Mathey-Prevot, B., Gonsalves, F., Changkakoty, B., & Perrimon, N. (2007). A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome Biology, 8(9), R203. https://doi.org/10.1186/gb-2007-8-9-r203
DasGupta, Ramanuj, Kent Nybakken, Matthew Booker, Bernard Mathey-Prevot, Foster Gonsalves, Binita Changkakoty, and Norbert Perrimon. “A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.Genome Biology 8, no. 9 (January 2007): R203. https://doi.org/10.1186/gb-2007-8-9-r203.
DasGupta R, Nybakken K, Booker M, Mathey-Prevot B, Gonsalves F, Changkakoty B, et al. A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome biology. 2007 Jan;8(9):R203.
DasGupta, Ramanuj, et al. “A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.Genome Biology, vol. 8, no. 9, Jan. 2007, p. R203. Epmc, doi:10.1186/gb-2007-8-9-r203.
DasGupta R, Nybakken K, Booker M, Mathey-Prevot B, Gonsalves F, Changkakoty B, Perrimon N. A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome biology. 2007 Jan;8(9):R203.

Published In

Genome biology

DOI

EISSN

1474-760X

ISSN

1474-7596

Publication Date

January 2007

Volume

8

Issue

9

Start / End Page

R203

Related Subject Headings

  • Transcription, Genetic
  • Sensitivity and Specificity
  • RNA, Double-Stranded
  • RNA Interference
  • RNA
  • Models, Genetic
  • Genome
  • Genetic Techniques
  • Genes, Insect
  • Gene Library